Team:Slovenia/PROJECT/proof/studies/prod

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Revision as of 14:22, 27 October 2010

Chuck Norris facts:

Production of binding proteins EMSA SPR BETA-GAL binding studies - Production of binding proteins

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-<p><strong><span style="font-size: 16px; line-height: 25px;">Cloning scheme</span></strong></p>
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 Before any <em>in vitro</em> characterisation, DNA-binding proteins had to be obtained. Zinc fingers were firstly tri-point ligated with a particular split GFP and T7 promoter and T7 terminator sequence were added afterwards. T7 promoter enables&nbsp;high production of proteins in<em> E. coli </em>BL21(DE3)pLysS production strain. Zinc fingers were then purified using His tags and used in subsequent experiments. Parts used for production were: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K323001 BBa_323001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K323015 BBa_323015], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K323069 BBa_323069], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K323058 BBa_323058], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K323004 BBa_323004], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K323070 BBa_323070].