Team:Slovenia/METHODS and PARTS/notebook/znf

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Revision as of 17:10, 27 October 2010

Fun fact:



week 1  week 11
week 2  week 12
week 3  week 13
week 4  week 14
week 5  week 15
week 6  week 16
week 7  week 17
week 8  week 18
week 9
week 10

5/31/2010 – 6/5/2010

DH5α cells were transformed with pSB1AK3 plasmid containing RBS-lacZ(full) insert and plated on LB with ampicylin (Amp). Single colonies were later inoculated into liquid LB media with Amp. Plasmid DNA was isolated from the over-night culture. The plasmid was cut with EcoRI and SpeI enzymes and the RBS-lacZ(full) fragment was isolated from agarose gel and ligated into pSB1AK3 vector containing DTER, which was previously cut with EcoRI and XbaI enzymes and isolated from the gel.