Team:Slovenia/METHODS and PARTS/notebook/viola


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Fun fact:

notebook - violacein

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DH5α cells were transformed with three plasmids (taken from the Registry) containing the violacein operon. (BBa_K274002, BBa_K274003,BBa_K274004). The cells were grown on LB Kan/Tc agar plates. Transformation of part Bba_K274002 was unsuccessful – there were no colonies on the LB Tc plates. Colonies containing plasmids with parts BBa_K274003 and BBa_K274004 were later inoculated into mini-prep flasks with Kan. Plasmid DNA was isolated from the over-night cultures.


Primers for amplificiation of separate vio genes were designed and ordered, and a table of constructs, necessary for the experiment was made.