Team:Slovenia/METHODS and PARTS/notebook/protein

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<a href="/Team:Slovenia/METHODS_and_PARTS/notebook"><span id="stopnja3" >Split/FRET</span></a>
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<a href="/Team:Slovenia/METHODS_and_PARTS/notebook/protein"><span id="stopnja3a">Proteins</span></a>
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<span id="naslov">notebook - proteins</span>
<span id="naslov">notebook - proteins</span>
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Latest revision as of 21:39, 27 October 2010

Fun fact:

notebook - proteins


week 1
week 2
week 3
week 4
week 5
week 6
week 7
week 8
week 9


8/23/2010 – 8/29/2010

 

Production of protein: cCFP_link_HivC (5 hours, 37°C, 160 RPM). Protein accumulated mainly in inclusion bodies  Cell lysates Protein analyzed by SDS-PAGE and western blot using antibodies agains His-Tag. Further protein purification of  protein in solubilized inclusion bodies was attempted by Ni-NTA column, and elution by increasing concentration of imidazole (25mM, 50mM, 100mM, 250mM, 500mM). After concentration using Centricon with the cutoff, the protein was re-analyzed by SDS page and Western blot.