Team:Slovenia/METHODS and PARTS/notebook/protein

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Revision as of 17:09, 27 October 2010

Fun fact:



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8/23/2010 – 8/29/2010

 

Production of protein: cCFP_link_HivC (5 hours, 37°C, 160 RPM). Protein accumulated mainly in inclusion bodies  Cell lysates Protein analyzed by SDS-PAGE and western blot using antibodies agains His-Tag. Further protein purification of  protein in solubilized inclusion bodies was attempted by Ni-NTA column, and elution by increasing concentration of imidazole (25mM, 50mM, 100mM, 250mM, 500mM). After concentration using Centricon with the cutoff, the protein was re-analyzed by SDS page and Western blot.