Team:Slovenia/METHODS and PARTS/notebook/carot
From 2010.igem.org
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<p><span style="font-family: Arial, sans-serif;">6/14/2010 –6/20/2010</span></p> | <p><span style="font-family: Arial, sans-serif;">6/14/2010 –6/20/2010</span></p> | ||
<p> </p> | <p> </p> | ||
- | <p><span style="font-family: Arial, sans-serif;">BioBrick parts [http://partsregistry.org/wiki/index.php/Part:BBa_K274100 BBa_K274100],[http://partsregistry.org/wiki/index.php/Part:BBa_K274110 BBa_K274110] ,[http://partsregistry.org/wiki/index.php/Part: BBa_K274120 BBa_K274120],[http://partsregistry.org/wiki/index.php/Part: BBa_K274200 BBa_K274200] ,[http://partsregistry.org/wiki/index.php/Part: BBa_K274210 BBa_K274210] and [http://partsregistry.org/wiki/index.php/Part: BBa_K274220 BBa_K274220] were transformed into E. coli DH5α. Plasmids were isolated from 10 mL overnight culture and analyzed by control restriction with enzymes EcoRI and PstI. Cultures with carotene and lycopene operons were inoculated in 10 mL LB medium with added arabinose for induction of pBAD promoter. Cultures were incubated for 24 h at 37°C and 160 rpm. Β-carotene and lycopene were extracted from cells with acetone, using the procedure from Cambridge 2009 iGEM team. VIS-spectra (400-550 nm) were recorded, which confirmed the production of B-carotene, but not lycopene </span></p> | + | <p><span style="font-family: Arial, sans-serif;">BioBrick parts [http://partsregistry.org/wiki/index.php/Part:BBa_K274100 BBa_K274100],[http://partsregistry.org/wiki/index.php/Part:BBa_K274110 BBa_K274110] ,[http://partsregistry.org/wiki/index.php/Part: BBa_K274120 BBa_K274120],[http://partsregistry.org/wiki/index.php/Part: BBa_K274200 BBa_K274200],[http://partsregistry.org/wiki/index.php/Part: BBa_K274210 BBa_K274210] and [http://partsregistry.org/wiki/index.php/Part: BBa_K274220 BBa_K274220] were transformed into E. coli DH5α. Plasmids were isolated from 10 mL overnight culture and analyzed by control restriction with enzymes EcoRI and PstI. Cultures with carotene and lycopene operons were inoculated in 10 mL LB medium with added arabinose for induction of pBAD promoter. Cultures were incubated for 24 h at 37°C and 160 rpm. Β-carotene and lycopene were extracted from cells with acetone, using the procedure from Cambridge 2009 iGEM team. VIS-spectra (400-550 nm) were recorded, which confirmed the production of B-carotene, but not lycopene </span></p> |
</div> | </div> | ||
Revision as of 17:36, 27 October 2010
Split/FRET
Oscillator
Violacein
Carotenoids
Proteins
Zinc-finger binding test
notebook - carotenoids
week 1week 11
week 2week 12
week 3week 13
week 4week 14
week 5week 15
week 6week 16
week 7week 17
week 8
week 9
week 10
6/7/2010 –6/13/2010
The following parts from registry were transformed into E. coli DH5α:
BBa_K152005, BBa_K118000,BBa_K118002,BBa_K118003,BBa_K118008,BBa_K118001,
BBa_K118004,BBa_K118007,BBa_K118014,BBa_I742158 .
Plasmids were isolated from 10 mL overnight culture and analyzed by control restriction with enzymes EcoRI and PstI. Plasmid pBAD crtEBIY GFP was constructed from BioBrick BBa_K118005 and pBAD/araC fragment.