Team:Slovenia/METHODS and PARTS/notebook

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Revision as of 21:00, 27 October 2010

Fun fact:

notebook - split/FRET


week 1  week 11
week 2  week 12
week 3  week 13
week 4  week 14
week 5  week 15
week 6  week 16
week 7  week 17
week 8  week 18
week 9  week 19
week 10week 20


6/17/2010 – 6/20/2010

 

PCR of C-terminal and N-terminal fragments of fluorescent proteins (termed split fluorescent proteins). mCerulean (cyan fluorescent protein) and mCitrine (yellow fluorescent protein) were used as  templates. N-splits were PCRed with reverse primer carrying stop codon, C-splits with forward primer carrying start codon and reverse primer carrying linker sequence.

 

reverse for N-splits: 5'-TGTACTGCAGGCGGCCGCACTAGTTTAGATGTTGTGGCGGATCTTG-3' 

forward for C-splits: 5'-ACTAGAATTCGCGGCCGCTCTAGAatgGCCGACAAGCAGAAGAACG-3' 

reverse for C-splits: 5'-TGTACTGCAGGCGGCCGCACTAGTGCTTCCCCCACTCCCACCGCCAGAGCCACCCTTGTACAGCTCGTCCATGC-3' 

EcoRI NotI XbaI SpeI PstI linker