Team:Slovenia/METHODS and PARTS/notebook

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<a href="/Team:Slovenia/METHODS_and_PARTS/notebook"><span id="stopnja3a" >Split/FRET</span></a>
 
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<a href="/Team:Slovenia/METHODS_and_PARTS/notebook/oscil"><span id="stopnja3">Oscillator</span></a>
 
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<a href="/Team:Slovenia/METHODS_and_PARTS/notebook/viola"><span id="stopnja3" margin-left:15px;">Violacein</span></a>
 
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<a href="/Team:Slovenia/METHODS_and_PARTS/notebook/carot"><span id="stopnja3" >Carotenoids</span></a>
 
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<a href="/Team:Slovenia/METHODS_and_PARTS/notebook/protein"><span id="stopnja3">Proteins</span></a>
 
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width:167px;" id="stopnja3">Zinc-finger binding test</span></a>
 
<span id="naslov">notebook - split/FRET </span>
<span id="naslov">notebook - split/FRET </span>
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<a href="/Team:Slovenia/METHODS_and_PARTS/notebook"><span id="stopnja3a" >split/FRET</span></a>
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<a href="/Team:Slovenia/METHODS_and_PARTS/notebook/oscil"><span id="stopnja3">oscillator</span></a>
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<a href="/Team:Slovenia/METHODS_and_PARTS/notebook/viola"><span id="stopnja3" margin-left:15px;">violacein</span></a>
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<a href="/Team:Slovenia/METHODS_and_PARTS/notebook/carot"><span id="stopnja3" >carotenoids</span></a>
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<a href="/Team:Slovenia/METHODS_and_PARTS/notebook/protein"><span id="stopnja3">proteins</span></a>
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<a href="/Team:Slovenia/METHODS_and_PARTS/notebook/znf"><span style="font-size:15px;
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width:167px;" id="stopnja3">zinc-finger binding test</span></a>
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<p>6/17/2010 – 6/20/2010</p>
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6/17/2010 – 6/20/2010
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<p>&nbsp;</p>
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<br>
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<p>PCR of C-terminal and N-terminal fragments of fluorescent proteins (termed split fluorescent proteins). mCerulean (cyan fluorescent protein) and mCitrine (yellow fluorescent protein) were used as &nbsp;templates. N-splits were PCRed with reverse primer carrying stop codon, C-splits with forward primer carrying start codon and reverse primer carrying linker sequence.</p>
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PCR of C-terminal and N-terminal fragments of fluorescent proteins (termed split fluorescent proteins). mCerulean (cyan fluorescent protein) and mCitrine (yellow fluorescent protein) were used as &nbsp;templates. N-splits were PCRed with reverse primer carrying stop codon, C-splits with forward primer carrying start codon and reverse primer carrying linker sequence.<br>
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<p>&nbsp;</p>
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<p><span style="font-size: 75%;">reverse for N-splits: 5'-TGTA<span style="background-color: rgb(128, 0, 128);">CTGCAG</span><span style="background-color: rgb(0, 255, 0);">GCGGCCGC</span><span style="background-color: rgb(255, 0, 255);">ACTAGT</span><span style="background-color: rgb(255, 255, 0);">TTA</span>GATGTTGTGGCGGATCTTG-3'</span>&nbsp;</p>
<p><span style="font-size: 75%;">reverse for N-splits: 5'-TGTA<span style="background-color: rgb(128, 0, 128);">CTGCAG</span><span style="background-color: rgb(0, 255, 0);">GCGGCCGC</span><span style="background-color: rgb(255, 0, 255);">ACTAGT</span><span style="background-color: rgb(255, 255, 0);">TTA</span>GATGTTGTGGCGGATCTTG-3'</span>&nbsp;</p>
<p><span style="font-size: 75%;">forward for C-splits: 5'-ACTA<span style="background-color: rgb(255, 0, 0);">GAATTC</span><span style="background-color: rgb(0, 255, 0);">GCGGCCGC</span><span style="background-color: rgb(0, 255, 255);">TCTAGA</span><span style="background-color: rgb(255, 255, 0);">atg</span>GCCGACAAGCAGAAGAACG-3'</span>&nbsp;</p>
<p><span style="font-size: 75%;">forward for C-splits: 5'-ACTA<span style="background-color: rgb(255, 0, 0);">GAATTC</span><span style="background-color: rgb(0, 255, 0);">GCGGCCGC</span><span style="background-color: rgb(0, 255, 255);">TCTAGA</span><span style="background-color: rgb(255, 255, 0);">atg</span>GCCGACAAGCAGAAGAACG-3'</span>&nbsp;</p>
<p><span style="font-size: 75%;">reverse for C-splits: 5'-TGTA<span style="background-color: rgb(128, 0, 128);">CTGCAG</span><span style="background-color: rgb(0, 255, 0);">GCGGCCGC</span><span style="background-color: rgb(255, 0, 255);">ACTAGT</span><span style="background-color: rgb(192, 192, 192);">GCTTCCCCCACTCCCACCGCCAGAGCCACC</span><span style="text-decoration: underline;">C</span></span><span style="font-size: 10px; line-height: 11px;">TTGTACAGCTCGTCCATGC-3'</span>&nbsp;</p>
<p><span style="font-size: 75%;">reverse for C-splits: 5'-TGTA<span style="background-color: rgb(128, 0, 128);">CTGCAG</span><span style="background-color: rgb(0, 255, 0);">GCGGCCGC</span><span style="background-color: rgb(255, 0, 255);">ACTAGT</span><span style="background-color: rgb(192, 192, 192);">GCTTCCCCCACTCCCACCGCCAGAGCCACC</span><span style="text-decoration: underline;">C</span></span><span style="font-size: 10px; line-height: 11px;">TTGTACAGCTCGTCCATGC-3'</span>&nbsp;</p>
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<p><span style="font-size: 75%; background-color: rgb(255, 0, 0);">EcoRI</span><span style="font-size: 75%;"> <span style="background-color: rgb(0, 255, 0);">NotI</span> <span style="background-color: rgb(0, 255, 255);">XbaI</span> <span style="background-color: rgb(255, 0, 255);">SpeI</span> <span style="background-color: rgb(128, 0, 128);">PstI</span> <span style="background-color: rgb(192, 192, 192);">linker</span>
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<p><span style="font-size: 75%; background-color: rgb(255, 0, 0);">EcoRI</span><span style="font-size: 75%;"> <span style="background-color: rgb(0, 255, 0);">NotI</span> <span style="background-color: rgb(0, 255, 255);">XbaI</span> <span style="background-color: rgb(255, 0, 255);">SpeI</span> <span style="background-color: rgb(128, 0, 128);">PstI</span> <span style="background-color: rgb(192, 192, 192);">linker</span></span></p>
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<p>&nbsp;</p>
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Latest revision as of 22:58, 27 October 2010

Fun fact:

notebook - split/FRET


week 1  week 11
week 2  week 12
week 3  week 13
week 4  week 14
week 5  week 15
week 6  week 16
week 7  week 17
week 8  week 18
week 9  week 19
week 10week 20


6/17/2010 – 6/20/2010
PCR of C-terminal and N-terminal fragments of fluorescent proteins (termed split fluorescent proteins). mCerulean (cyan fluorescent protein) and mCitrine (yellow fluorescent protein) were used as  templates. N-splits were PCRed with reverse primer carrying stop codon, C-splits with forward primer carrying start codon and reverse primer carrying linker sequence.

reverse for N-splits: 5'-TGTACTGCAGGCGGCCGCACTAGTTTAGATGTTGTGGCGGATCTTG-3' 

forward for C-splits: 5'-ACTAGAATTCGCGGCCGCTCTAGAatgGCCGACAAGCAGAAGAACG-3' 

reverse for C-splits: 5'-TGTACTGCAGGCGGCCGCACTAGTGCTTCCCCCACTCCCACCGCCAGAGCCACCCTTGTACAGCTCGTCCATGC-3' 

EcoRI NotI XbaI SpeI PstI linker