Team:SJTU-BioX-Shanghai

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Hello everyone, we're igemers from team SJTU-BioX-Shanghai and we're glad to present our project to you!

Project description:

Synthetic-biological Approaches to Osteoarthritis

Osteoarthritis (OA) is a chronic disease in which joint matrix is degraded and chondrocytes undergo disordered and hypertrophic differentiation, symptoms including joint pain, tenderness and stiffness. We proposed two synthetic-biological approaches to OA, one with a eukaryotic genetic circuit and another prokaryotic. Both circuits are composed of three systems: "Detector", "Actuator" and "Supervisor". As for Detector, we built tissue-specific promoters in the eukaryotic circuit, while inflammation factors are employed as signals of OA in the prokaryotic circuit. The same Actuator shared by two circuits generates proteins col2a1, which replenishes the degraded matrix, and oct4, which reverses the disordered differentiation. The eukaryotic Supervisor part has an original design in which a photo-sensitive cation channel crosstalks with certain cellular signaling pathways, resulting in the light-controlled expression of col2a1 and oct4; in the counterpart of prokaryotic circuit, both injected inducers and over-population lead the engineered bacteria to suicide, thus attenuating possible side effects.

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-Hello everyone, we're igemers from [[Team:SJTU-BioX-Shanghai/team|team SJTU-BioX-Shanghai]] and we're glad to present our projects to you! At the moment we have 2 projects developing in our lab, and both of them are progressing smoothly.
+Hello everyone, we're igemers from [[Team:SJTU-BioX-Shanghai/team|team SJTU-BioX-Shanghai]] and we're glad to present our project to you!
-===Project descriptions:===
-====1. Light-controlled tumor therapy====
+===Project description:===
-We have designed our project as a 'light-controlled tumor therapy', which might prove an innovative treatment for tumor with light as its 'switch'. Viral vectors deliver our eukaryotic genetic circuit into tumor cells. Light-controlled cation channels are expressed and then activated by exposure to light of a specific wavelength, and the influx of cations 'switches on' a downstream effector device, which brings about necrosis and apoptosis exclusively within tumor cells. Normal cells are protected by a dual lock — tumor-specific promoter of the whole circuit and inhibitor protein of effector which avails when cells are not exposed to specific light.
-====2. An synthetic biological approach to osteoarthritis(OA)====
+====Synthetic-biological Approaches to Osteoarthritis====
-We try to construct a kind of genetically engineered bacteria which can detect the osteoarthritis (OA) and then repair the  articular cartilage suffered from OA.
+Osteoarthritis (OA) is a chronic disease in which joint matrix is degraded and chondrocytes undergo disordered and hypertrophic differentiation, symptoms including joint pain, tenderness and stiffness. We proposed two synthetic-biological approaches to OA, one with a eukaryotic genetic circuit and another prokaryotic. Both circuits are composed of three systems: "Detector", "Actuator" and "Supervisor". As for Detector, we built tissue-specific promoters in the eukaryotic circuit, while inflammation factors are employed as signals of OA in the prokaryotic circuit. The same Actuator shared by two circuits generates proteins col2a1, which replenishes the degraded matrix, and oct4, which reverses the disordered differentiation. The eukaryotic Supervisor part has an original design in which a photo-sensitive cation channel crosstalks with certain cellular signaling pathways, resulting in the light-controlled expression of col2a1 and oct4; in the counterpart of prokaryotic circuit, both injected inducers and over-population lead the engineered bacteria to suicide, thus attenuating possible side effects.
-Frequent heavy burden on cartilage will cause damage. If the damage are left untreated, the damaged joint will progress to osteoarthritic cartilage, i.e. cartilage suffered from OA.
-In OA,  chondrocytes in resting state will abnormally differentiate. Meanwhile, the matrix of joint is degrading. In order to treat OA, we try to reverse the differentiation of chondrocytes and supplement the matrix. Therefore, we decide to construct genetically engineered bacteria that have these two abilities and can be used as a kind of therapy to cure OA.
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