Team:SDU-Denmark/safety-b

From 2010.igem.org

(Difference between revisions)
(Risk-assessment for Individual Parts)
(Risk-assessment for Individual Parts)
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The BioBrick’s product is not in itself toxic, but we do not recommend using this BioBrick for any type of system in humans or animals for the following reasons:  
The BioBrick’s product is not in itself toxic, but we do not recommend using this BioBrick for any type of system in humans or animals for the following reasons:  
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*Retinoic acid, which retinal can degrade into, can affect gene expression and function of almost any cell, including cells of the immune system; it also plays a fundamental role in cellular functions by activating nuclear receptors (1).  
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*Retinoic acid, which retinal can degrade into, can affect gene expression and function of almost any cell, including cells of the immune system; it also plays a fundamental role in cellular functions by activating nuclear receptors ([http://2010.igem.org/Team:SDU-Denmark/safety-b#References 1]).  
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*Vitamin A toxicity can lead to hepatic congestion and fibrosis (2).  
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*Vitamin A toxicity can lead to hepatic congestion and fibrosis ([http://2010.igem.org/Team:SDU-Denmark/safety-b#References 2]).  
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*Vitamin A and its derivatives have been implicated as chemopreventive and differentiating agents in a variety of cancers (3).  
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*Vitamin A and its derivatives have been implicated as chemopreventive and differentiating agents in a variety of cancers ([http://2010.igem.org/Team:SDU-Denmark/safety-b#References 3]).  
These effects are not directly associated with the enzyme itself, but have been observed in humans. It is highly unlikely that high enough doses can be reached with this biobrick. Please see references for more information about the diseases.  
These effects are not directly associated with the enzyme itself, but have been observed in humans. It is highly unlikely that high enough doses can be reached with this biobrick. Please see references for more information about the diseases.  
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The biobrick should not increase its host’s ability to spread, survive outside the laboratory, and will most likely decrease its ability to replicate.
The biobrick should not increase its host’s ability to spread, survive outside the laboratory, and will most likely decrease its ability to replicate.
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Beta-caroten monooxygenase is found in a wide variety of different bacteria, insects and animals (4). As such we would be cautious as to letting a system containing this BioBrick into the wild, since it's function might conflict with existing systems. On the other hand one might argue that since it's function is already available in nature, the function is widely available.  
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Beta-caroten monooxygenase is found in a wide variety of different bacteria, insects and animals ([http://2010.igem.org/Team:SDU-Denmark/safety-b#References 4]). As such we would be cautious as to letting a system containing this BioBrick into the wild, since it's function might conflict with existing systems. On the other hand one might argue that since it's function is already available in nature, the function is widely available.  
The product of the BioBrick, retinal, also plays an important function in nature and animals. For this reason we fell that the BioBrick could be used in controlled settings, but not in the wild.  
The product of the BioBrick, retinal, also plays an important function in nature and animals. For this reason we fell that the BioBrick could be used in controlled settings, but not in the wild.  
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====Potential pathogenicity====
====Potential pathogenicity====
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This biobrick increases the potential of its host to move. Increased motility has been associated the bacteria’s ability to invade humans (5); on the other hand it has also been shown that bacteria that loss the function of the FlhDC operon, are considerable better at colonizing the intestine (6), and so an increased expression might decrease a hosts ability to colonize and invade humans. It is impossible to ensure, that this plasmid is not transferred to pathogenic bacteria since the FlhDC operons is used in a wide array of other bacteria that are known to be pathogenic.
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This biobrick increases the potential of its host to move. Increased motility has been associated the bacteria’s ability to invade humans ([http://2010.igem.org/Team:SDU-Denmark/safety-b#References 5]); on the other hand it has also been shown that bacteria that loss the function of the FlhDC operon, are considerable better at colonizing the intestine ([http://2010.igem.org/Team:SDU-Denmark/safety-b#References 6]), and so an increased expression might decrease a hosts ability to colonize and invade humans. It is impossible to ensure, that this plasmid is not transferred to pathogenic bacteria since the FlhDC operons is used in a wide array of other bacteria that are known to be pathogenic.
A number of effector cells in the human immune system react specifically to bacteria’s flagella, and so a hyperflagellated host will most likely induce a stronger immune response.  
A number of effector cells in the human immune system react specifically to bacteria’s flagella, and so a hyperflagellated host will most likely induce a stronger immune response.  
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====Potential pathogenicity====
====Potential pathogenicity====
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This BioBrick consists of three different parts: The first 224 amino acid residues come from the NpSopII gene from halobacteria, encoding a blue-light photon receptor with 15 residues removed at the C-terminal. The following 9 amino acids are a linker. The last part is HtrII fused with Tsr from E. Coli. The complex' first 125 amino acid residues come from HtrII and the remaining 279 from Tsr (7). NpHtrII is thought to function in signal transduction and activation of microbial signalling cascades (8).   
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This BioBrick consists of three different parts: The first 224 amino acid residues come from the NpSopII gene from halobacteria, encoding a blue-light photon receptor with 15 residues removed at the C-terminal. The following 9 amino acids are a linker. The last part is HtrII fused with Tsr from E. Coli. The complex' first 125 amino acid residues come from HtrII and the remaining 279 from Tsr ([http://2010.igem.org/Team:SDU-Denmark/safety-b#References 7]). NpHtrII is thought to function in signal transduction and activation of microbial signalling cascades ([http://2010.igem.org/Team:SDU-Denmark/safety-b#References 8]).   
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A single article has been written about haloarchaea in humans indicating that these played a role in patients with inflammatory bowl disease (9), but there is no evidence that the genes or near homologs, this BioBrick is made from, are involved in any disease process, toxic products or invasion properties. They do not regulate the immune system in any way.
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A single article has been written about haloarchaea in humans indicating that these played a role in patients with inflammatory bowl disease ([http://2010.igem.org/Team:SDU-Denmark/safety-b#References 9]), but there is no evidence that the genes or near homologs, this BioBrick is made from, are involved in any disease process, toxic products or invasion properties. They do not regulate the immune system in any way.
====Environmental impact====
====Environmental impact====
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''Is your construct watermarked?''
''Is your construct watermarked?''
No.
No.
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===References===
===References===

Revision as of 11:26, 26 October 2010