Team:SDU-Denmark/protocols

From 2010.igem.org

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3. Resuspend cells in 8 mL acetone and sonicate the sample for 2x 30 sek<br> <br>
3. Resuspend cells in 8 mL acetone and sonicate the sample for 2x 30 sek<br> <br>
4. Centrifuge the samples at 16000g for 2 min and collect 2 mL of the supernatant<br><br>
4. Centrifuge the samples at 16000g for 2 min and collect 2 mL of the supernatant<br><br>
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5. Measure absorbance using UV-Vis spectrophotometer at 450 nm, was preformed on a …. From [http://www.memphys.sdu.dk/ MEMPHYS]<br><br>
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5. Measure absorbance using UV-Vis spectrophotometer at 450 nm  
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Get a template for calculating ligation concentrations [https://static.igem.org/mediawiki/2010/4/40/Team_SDUTemplate_for_ligation.ZIP here] <br><br><br>
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Almost like cake recipes... although cake is infinitely more delicious than bacteria. <br><br>
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Almost like cake recipes... although cake is infinitely more delicious than bacteria.
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Get a template for calculating your ligation concentrations <br><html><a href="https://static.igem.org/mediawiki/2010/4/40/Team_SDUTemplate_for_ligation.ZIP" style="color: #50CC38; text-decoratio: bold; font-size: 24px;">HERE</a></html>
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[https://2010.igem.org/Team:SDU-Denmark/project/activities/commoninterest/diller 8===D]
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Latest revision as of 03:58, 28 October 2010