Team:SDU-Denmark/protocols

From 2010.igem.org

(Difference between revisions)
(PS1.1)
(GA1.1)
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1. 5mL LB media containing the appropriate antibiotic is inoculated with a colony .<br>
1. 5mL LB media containing the appropriate antibiotic is inoculated with a colony .<br>
2. The culture is incubated over night at 37°C and 180rpm.<br>
2. The culture is incubated over night at 37°C and 180rpm.<br>
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3. The optical density at 550nm (OD550) of the overnight culture is measured and the culture is diluted in 25uL fresh LB media containing the appropriate antibiotic to reach an OD550 of 0.02. The culture is then incubated at 37°C and 180rpm.<br>
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3. The optical density at 550nm (OD550) of the overnight culture is measured and the culture is diluted in 15mL fresh LB media containing the appropriate antibiotic to reach an OD550 of 0.02. The culture is then incubated at 37°C and 180rpm.<br>
4. OD550 of the colony is measured every hour for the first 12 hours, and after 24 hours.<br><br>  
4. OD550 of the colony is measured every hour for the first 12 hours, and after 24 hours.<br><br>  

Revision as of 19:41, 23 October 2010