Team:SDU-Denmark/protocols

From 2010.igem.org

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(SEM 1.1)
(SEM 1.1)
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Day 1: The bacteria was cultivated overnight in 5 ml LB-media at 37 degrees celcius. <br><br>  
Day 1: The bacteria was cultivated overnight in 5 ml LB-media at 37 degrees celcius. <br><br>  
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Day 2: The overnight culture was centrifuged  15 min at 4000prm. Afterwards the pellet was resuspended in  distilled water. We aimed to get approximately 106 bacteria in 10 µl solution which was plated on double adhesive tape at the top of the grid. The solution was allowed to air dry and the remaining fluid disappeared when sample were exposed to the vacuum in the electron microscope.  <br><br>
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Day 2: The overnight culture was centrifuged  15 min at 4000prm. Afterwards the pellet was resuspended in  distilled water. We aimed to get approximately 10<sup>6</sup> bacteria in 10 µl solution which was plated on double adhesive tape at the top of the grid. The solution was allowed to air dry and the remaining fluid disappeared when sample were exposed to the vacuum in the electron microscope.  <br><br>
The sample was examined with different electron intensity and magnification. We found that the best picture was taken with a electron intensity of 10 kv.
The sample was examined with different electron intensity and magnification. We found that the best picture was taken with a electron intensity of 10 kv.

Revision as of 18:53, 23 October 2010