Team:SDU-Denmark/protocols

From 2010.igem.org

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(SA 1.1)
(SA 1.1)
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2.      The culture is incubated over night at 30°C and 180rpm
2.      The culture is incubated over night at 30°C and 180rpm
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3.      100µL of the culture is serial diluted in 900µL 0.9% NaCl until a dilution of 10<sup>7</sup> is reached.
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3.      100µL of the culture is serially diluted in 900µL 0.9% NaCl until a dilution of 10<sup>7</sup> is reached.
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4.      100µL of the 10<sup>5</sup>, 10<sup>6</sup> and 10<sup>7</sup> dilutiona are spread onto LA plates, and LA plates with the appropriate antibiotic, respectively.
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4.      100µL of the 10<sup>5</sup>, 10<sup>6</sup> and 10<sup>7</sup> dilutions are spread onto LA plates, and LA plates with the appropriate antibiotic, respectively.
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5.      Plates are incubated at 37°C for 16-24 hours. The following day the colonies formed on the plates are counted and cfu are determined.
5.      Plates are incubated at 37°C for 16-24 hours. The following day the colonies formed on the plates are counted and cfu are determined.

Revision as of 18:51, 23 October 2010