Team:SDU-Denmark/protocols

From 2010.igem.org

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(AG1.1)
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=== EX1.1 ===
=== EX1.1 ===
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1. Incubate E. Coli in 110 ml LB media with appropriate antibiotics at 37 oC for 20 hours<br>  
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1. Incubate ''E. Coli'' in 110 ml LB media with appropriate antibiotics at 37 oC for 20 hours<br>  
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2. Harvest cells using centrifugation at 4000 G for 15 min<br>
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2. Harvest cells by centrifugation at 4000 G for 15 min<br>
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3. Re-suspend cells in 8 mL acetone and sonicate the sample for 2x 30 sek<br>  
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3. Resuspend cells in 8 mL acetone and sonicate the sample for 2x 30 sek<br>  
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4. Centrifuge the samples at 16000 G for 2 min and collect 2 mL of the supernatant<br>
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4. Centrifuge the samples at 16000g for 2 min and collect 2 mL of the supernatant<br>
5. Measure absorbance using UV-Vis spectrophotometer at 450 nm, was preformed on a …. From MEMPHYS<br>
5. Measure absorbance using UV-Vis spectrophotometer at 450 nm, was preformed on a …. From MEMPHYS<br>
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=== EX1.2 ===
=== EX1.2 ===
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Revision as of 16:58, 23 October 2010