Team:SDU-Denmark/protocols

From 2010.igem.org

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8.      The experiment is carried out for 5 days. (NB: antibiotic is only added to the first culture. The remaining days the bacteria are grown in cultures without any antibiotics)
8.      The experiment is carried out for 5 days. (NB: antibiotic is only added to the first culture. The remaining days the bacteria are grown in cultures without any antibiotics)
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==Scanning Electron Microscope ==
===SEM1.1===
===SEM1.1===
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Scanning electron microscope <br><br>
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Day 1: The bacteria was cultivated ON in 5 ml LB-media at 37 degrees. <br><br>  
Day 1: The bacteria was cultivated ON in 5 ml LB-media at 37 degrees. <br><br>  
Day 2: The ON culture was centrifuged  15 min at 4000prm. Afterwards the pellet was resuspended in  distilled water. We aimed to get approximately 106 bacteria in 10 µl solution which was plated on double adhesive tape at the top of the grid. The solution was allowed to air dry and the remaining fluid appeared when sample were exposed to the vacuum in the electron microscope.  <br><br>
Day 2: The ON culture was centrifuged  15 min at 4000prm. Afterwards the pellet was resuspended in  distilled water. We aimed to get approximately 106 bacteria in 10 µl solution which was plated on double adhesive tape at the top of the grid. The solution was allowed to air dry and the remaining fluid appeared when sample were exposed to the vacuum in the electron microscope.  <br><br>

Revision as of 19:40, 22 October 2010