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Team:SDU-Denmark/project-r
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'''Photosensor'''<br> | '''Photosensor'''<br> | ||
The results of the motility assay are just in. In this experiment we shone blue light on one half of the plate, while the other half was in the dark. We then placed one colony in each half and observed their motility pattern after 24 hours. We did this for three different strains of E.Coli - DH5alpha, Wildtype Mg1655 and Mg1655 containing a plasmid with our photosensor constitutively on. See the results for yourself:<br> | The results of the motility assay are just in. In this experiment we shone blue light on one half of the plate, while the other half was in the dark. We then placed one colony in each half and observed their motility pattern after 24 hours. We did this for three different strains of E.Coli - DH5alpha, Wildtype Mg1655 and Mg1655 containing a plasmid with our photosensor constitutively on. See the results for yourself:<br> | ||
| + | Light shone on the right half of the plates, the left half was in the dark.<br> | ||
<br>Mg1655 (Wildtype): | <br>Mg1655 (Wildtype): | ||
[[Image:Team-SDU-Denmark-MG1655.JPG|400px]]<br> | [[Image:Team-SDU-Denmark-MG1655.JPG|400px]]<br> | ||
<br>Photosensor (Modified Mg1655) | <br>Photosensor (Modified Mg1655) | ||
| - | [[Image: | + | [[Image:Team-SDU-Denmark-Photosensor.JPG|400px]]<br> |
<br>DH5alpha (Reduced motility): | <br>DH5alpha (Reduced motility): | ||
[[Image:Team-SDU-Denmark-DH5alpha.JPG|400px]]<br> | [[Image:Team-SDU-Denmark-DH5alpha.JPG|400px]]<br> | ||
Revision as of 17:05, 29 September 2010
Results
Photosensor
The results of the motility assay are just in. In this experiment we shone blue light on one half of the plate, while the other half was in the dark. We then placed one colony in each half and observed their motility pattern after 24 hours. We did this for three different strains of E.Coli - DH5alpha, Wildtype Mg1655 and Mg1655 containing a plasmid with our photosensor constitutively on. See the results for yourself:
Light shone on the right half of the plates, the left half was in the dark.
Mg1655 (Wildtype):
Photosensor (Modified Mg1655)
DH5alpha (Reduced motility):
As you can see, both colonies on the photosensor plate moved towards the darker end of their plate, trying to get awaz from the light. Because of the increased tumbling the colony in the light did not move very far, but the little movement that happened was away from the light. On the dark half, the colony moved alot more, because of the decreased tumbling frequency. This is confirmed by the movement towards the darker edge of the plate.
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