Team:SDU-Denmark/project-r

From 2010.igem.org

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=== Effect ===
=== Effect ===
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Our results from the experiments with semi-solid agar confirm that the biobrick does indeed couple itself to the bacterial chemotaxis pathway and modify the bacterial motility pattern by modifying the tumbling frequency. For further information on this conclusion see [http://2010.igem.org/Team:SDU-Denmark/project-p#Growth_of_bacterial_culture_on_semi-solid_agar_plates growth of bacteria on semi-solid agar plates]<br>
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Our results from the experiments with semi-solid agar confirm that the biobrick does indeed couple itself to the bacterial chemotaxis pathway and modify the bacterial motility pattern by modifying the tumbling frequency.<br>
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The videomicroscopy indicates that blue light with a wavelength around 500nm leads to CheA's autophosphorylation being downregulated. This means that the bacterial tumbling frequency gets reduced and the bacteria will spend an increased time in the "run" mode of propulsion. This means that the bacteria will travel further in a certain amount of time, compared to wildtype bacteria. More details can be found here [http://2010.igem.org/Team:SDU-Denmark/project-p#Videomicroscopy_and_computer_analysis_of_bacterial_motility videomicroscopy]<br>
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The videomicroscopy indicates that blue light with a wavelength around 500nm leads to CheA's autophosphorylation being downregulated. This means that the bacterial tumbling frequency gets reduced and the bacteria will spend an increased time in the "run" mode of propulsion. This means that the bacteria will travel further in a certain amount of time, compared to wildtype bacteria.<br>
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The stability of pSB1C3-K343007 is most likely <20 generations, however the stability of pSB3T5-K343007 was not determined (see [http://2010.igem.org/Team:SDU-Denmark/project-p#Stability_assay stability assay])<br><br>
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The stability of pSB1C3-K343007 is most likely <20 generations, however the stability of pSB3T5-K343007 was not determined <br>
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Plasmids expressing K343007 does not seem to hinder the bacterial growth in any way (see [http://2010.igem.org/Team:SDU-Denmark/project-p#Growth_assay growth assay])<br><br>
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Plasmids expressing K343007 does not seem to hinder the bacterial growth in any way<br>
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For further information, raw data and background of the assay see [http://2010.igem.org/Team:SDU-Denmark/project-p#K343007 characterization of K343007]
==Flagella==
==Flagella==

Revision as of 12:39, 26 October 2010