Team:SDU-Denmark/project-r

From 2010.igem.org

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Our results from the experiments with semi-solid agar confirm that the biobrick does indeed couple itself to the bacterial chemotaxis pathway and modify the bacterial motility pattern by modifying the tumbling frequency. For further information on this conclusion see [http://2010.igem.org/Team:SDU-Denmark/project-p#K343007| Characterization - K343007 - 1. Growth of bacterial culture on semi-solid agar plates (Experiment 1 and 2)]<br>
Our results from the experiments with semi-solid agar confirm that the biobrick does indeed couple itself to the bacterial chemotaxis pathway and modify the bacterial motility pattern by modifying the tumbling frequency. For further information on this conclusion see [http://2010.igem.org/Team:SDU-Denmark/project-p#K343007| Characterization - K343007 - 1. Growth of bacterial culture on semi-solid agar plates (Experiment 1 and 2)]<br>
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The videomicroscopy indicates that blue light with a wavelength around 500nm leads to CheA's autophosphorylation being downregulated. This means that the bacterial tumbling frequency gets reduced and the bacteria will spend an increased time in the "run" mode of propulsion. This means that the bacteria will travel further in a certain amount of time, compared to wildtype bacteria. More details can be found here [http://2010.igem.org/Team:SDU-Denmark/project-p#K343007| Characterization - K343007 - 2. Videomicroscopy and computer analysis of bacterial motility]<br>
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The videomicroscopy indicates that blue light with a wavelength around 500nm leads to CheA's autophosphorylation being downregulated. This means that the bacterial tumbling frequency gets reduced and the bacteria will spend an increased time in the "run" mode of propulsion. This means that the bacteria will travel further in a certain amount of time, compared to wildtype bacteria. More details can be found here [[http://2010.igem.org/Team:SDU-Denmark/project-p#Growth_of_bacterial_culture_on_semi-solid_agar_plates characterization of K343007]]<br>
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The growth assay showed no significant difference between the wild type and the cells transformed with the biobrick.
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The stability of pSB1C3-K343007 is most likely <20 generations, however the stability of pSB3T5-K343007 was not determined (see [[http://2010.igem.org/Team:SDU-Denmark/project-p#Stability_assay characterization of K343007]])<br><br>
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Regarding the stability of the plasmid, we carried out a stability assay according to protocol [http://2010.igem.org/Team:SDU-Denmark/protocols#SA_1.1|SA1.1] There was no observeable difference between the cultures plated out on plates with and without antibiotics. From this we conclude that the plasmid does not significantly increase the stress on the bacteria, since they don't seem to lose the plasmid after 5 days. Since this did not happen the plasmid seems to be stable. For a closer look at the results look here:
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Plasmids expressing K343007 does not seem to hinder the bacterial growth in any way (see [[http://2010.igem.org/Team:SDU-Denmark/project-p#Growth_assay characterization of K343007]])<br>
== Retinal ==
== Retinal ==

Revision as of 12:08, 26 October 2010