Team:SDU-Denmark/project-r

From 2010.igem.org

(Difference between revisions)
(Stability assay)
(Effect)
Line 16: Line 16:
When comparing the sequence that came back from sequencing with the theoretical sequence the part should have it is identical, except for a silent mutation in the coding sequence, which does not change the amino acid sequence.
When comparing the sequence that came back from sequencing with the theoretical sequence the part should have it is identical, except for a silent mutation in the coding sequence, which does not change the amino acid sequence.
-
=== Effect ===
+
 
-
Our results from the experiments with semi-solid agar confirm that the biobrick does indeed couple itself to the bacterial chemotaxis pathway and modify the bacterial motility pattern by modifying the tumbling frequency. For further information on this conclusion see [http://2010.igem.org/Team:SDU-Denmark/project-p#K343007| Characterization - K343007 - 1. Growth of bacterial culture on semi-solid agar plates (Experiment 1 and 2)]<br>
+
-
<br>
+
-
The videomicroscopy indicates that blue light with a wavelength around 500nm leads to CheA's autophosphorylation being downregulated. This means that the bacterial tumbling frequency gets reduced and the bacteria will spend an increased time in the "run" mode of propulsion. This means that the bacteria will travel further in a certain amount of time, compared to wildtype bacteria. More details can be found here [http://2010.igem.org/Team:SDU-Denmark/project-p#K343007| Characterization - K343007 - 2. Videomicroscopy and computer analysis of bacterial motility]<br>
+
-
<br>
+
=== Effect ===
=== Effect ===

Revision as of 20:14, 24 October 2010