Team:SDU-Denmark/project-r

From 2010.igem.org

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(Stability assay)
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Regarding the stability of the plasmid, we carried out a stability assay according to protocol [http://2010.igem.org/Team:SDU-Denmark/protocols#SA_1.1|SA1.1] There was no observeable difference between the cultures plated out on plates with and without antibiotics. From this we conclude that the plasmid does not significantly increase the stress on the bacteria, since they don't seem to lose the plasmid after 5 days. Since this did not happen the plasmid seems to be stable. For a closer look at the results look here:
Regarding the stability of the plasmid, we carried out a stability assay according to protocol [http://2010.igem.org/Team:SDU-Denmark/protocols#SA_1.1|SA1.1] There was no observeable difference between the cultures plated out on plates with and without antibiotics. From this we conclude that the plasmid does not significantly increase the stress on the bacteria, since they don't seem to lose the plasmid after 5 days. Since this did not happen the plasmid seems to be stable. For a closer look at the results look here:
-
=== Stability assay ===
 
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<head>
 
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  <meta content="text/html; charset=ISO-8859-1"
 
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http-equiv="content-type">
 
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  <title></title>
 
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<body>
 
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<table x:str=""
 
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style="border-collapse: collapse; width: 428pt;" border="0"
 
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cellpadding="0" cellspacing="0" width="570">
 
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  <col style="width: 75pt; font-weight: bold;" width="100">
 
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  <col style="width: 79pt; font-weight: bold;" width="105">
 
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  <col style="width: 128pt; font-weight: bold;" width="170">
 
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  <col style="width: 53pt; font-weight: bold;" width="71">
 
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  <col style="width: 93pt; font-weight: bold;" width="124">
 
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  <tbody>
 
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    <tr style="height: 15pt;" height="20">
 
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      <td class="xl65"
 
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style="height: 15pt; width: 75pt; font-weight: bold;"
 
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height="20" width="100">Stability assay.</td>
 
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      <td class="xl65" style="width: 79pt;" width="105"></td>
 
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      <td style="width: 128pt;" width="170"></td>
 
-
      <td style="width: 53pt;" width="71"></td>
 
-
      <td style="width: 93pt;" width="124"></td>
 
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    </tr>
 
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    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20"></td>
 
-
      <td></td>
 
-
      <td></td>
 
-
      <td></td>
 
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      <td></td>
 
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    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td class="xl65"
 
-
style="height: 15pt; text-decoration: underline;" height="20">0
 
-
generations</td>
 
-
      <td style="font-weight: bold;">Dilution number</td>
 
-
      <td style="font-weight: bold;">Colonies counted on
 
-
plate</td>
 
-
      <td style="font-weight: bold;">Cfu</td>
 
-
      <td style="font-weight: bold;">PS-chlor/PS+chlor</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS1 +
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">168</td>
 
-
      <td x:num="8400000000" x:fmla="=(10^B4)*C4*50"
 
-
align="right">8.4E+09</td>
 
-
      <td x:num="" align="right">100</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS1 -
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">157</td>
 
-
      <td x:num="7850000000" x:fmla="=(10^B5)*C5*50"
 
-
align="right">7.85E+09</td>
 
-
      <td></td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS2 +
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">192</td>
 
-
      <td x:num="9600000000" x:fmla="=(10^B6)*C6*50"
 
-
align="right">9.6E+09</td>
 
-
      <td x:num="" align="right">100</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS2 -
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">216</td>
 
-
      <td x:num="10800000000" x:fmla="=(10^B7)*C7*50"
 
-
align="right">1.08E+10</td>
 
-
      <td></td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td class="xl65"
 
-
style="height: 15pt; text-decoration: underline;" height="20">20
 
-
generations</td>
 
-
      <td class="xl65"></td>
 
-
      <td></td>
 
-
      <td></td>
 
-
      <td></td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS1 +
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">157</td>
 
-
      <td x:num="7850000000" x:fmla="=(10^B9)*C9*50"
 
-
align="right">7.85E+09</td>
 
-
      <td x:num="59.245283018867923" x:fmla="=(D9/D10)*100"
 
-
align="right">59.24528302</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS1 -
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">265</td>
 
-
      <td x:num="13250000000" x:fmla="=(10^B10)*C10*50"
 
-
align="right">1.325E+10</td>
 
-
      <td></td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS2 +
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">130</td>
 
-
      <td x:num="6500000000" x:fmla="=(10^B11)*C11*50"
 
-
align="right">6.5E+09</td>
 
-
      <td x:num="63.414634146341463" x:fmla="=(D11/D12)*100"
 
-
align="right">63.41463415</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS2 -
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">205</td>
 
-
      <td x:num="10250000000" x:fmla="=(10^B12)*C12*50"
 
-
align="right">1.025E+10</td>
 
-
      <td></td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td class="xl65"
 
-
style="height: 15pt; text-decoration: underline;" height="20">40
 
-
generations</td>
 
-
      <td class="xl65"></td>
 
-
      <td></td>
 
-
      <td></td>
 
-
      <td></td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS1 +
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">143</td>
 
-
      <td x:num="7150000000" x:fmla="=(10^B14)*C14*50"
 
-
align="right">7.15E+09</td>
 
-
      <td x:num="95.973154362416096" x:fmla="=(D14/D15)*100"
 
-
align="right">95.97315436</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS1 -
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">149</td>
 
-
      <td x:num="7450000000" x:fmla="=(10^B15)*C15*50"
 
-
align="right">7.45E+09</td>
 
-
      <td></td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS2 +
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">168</td>
 
-
      <td x:num="8400000000" x:fmla="=(10^B16)*C16*50"
 
-
align="right">8.4E+09</td>
 
-
      <td x:num="92.817679558011051" x:fmla="=(D16/D17)*100"
 
-
align="right">92.81767956</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS2 -
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">181</td>
 
-
      <td x:num="9050000000" x:fmla="=(10^B17)*C17*50"
 
-
align="right">9.05E+09</td>
 
-
      <td></td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td class="xl65"
 
-
style="height: 15pt; text-decoration: underline;" height="20">60
 
-
generations</td>
 
-
      <td style="text-decoration: underline;" class="xl65"></td>
 
-
      <td style="text-decoration: underline;"></td>
 
-
      <td style="text-decoration: underline;"></td>
 
-
      <td style="text-decoration: underline;"></td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS1 +
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">147</td>
 
-
      <td x:num="7350000000" x:fmla="=(10^B19)*C19*50"
 
-
align="right">7.35E+09</td>
 
-
      <td x:num="89.634146341463421" x:fmla="=(D19/D20)*100"
 
-
align="right">89.63414634</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS1 -
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">164</td>
 
-
      <td x:num="8200000000" x:fmla="=(10^B20)*C20*50"
 
-
align="right">8.2E+09</td>
 
-
      <td></td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS2 +
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">134</td>
 
-
      <td x:num="6700000000" x:fmla="=(10^B21)*C21*50"
 
-
align="right">6.7E+09</td>
 
-
      <td x:num="104.6875" x:fmla="=(D21/D22)*100"
 
-
align="right">104.6875</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS2 -
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">128</td>
 
-
      <td x:num="6400000000" x:fmla="=(10^B22)*C22*50"
 
-
align="right">6.4E+09</td>
 
-
      <td></td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td class="xl65"
 
-
style="height: 15pt; text-decoration: underline;" height="20">80
 
-
generations</td>
 
-
      <td class="xl65"></td>
 
-
      <td></td>
 
-
      <td></td>
 
-
      <td></td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS1 +
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">127</td>
 
-
      <td x:num="6350000000" x:fmla="=(10^B24)*C24*50"
 
-
align="right">6.35E+09</td>
 
-
      <td x:num="136.55913978494624" x:fmla="=(D24/D25)*100"
 
-
align="right">136.5591398</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS1 -
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">93</td>
 
-
      <td x:num="4650000000" x:fmla="=(10^B25)*C25*50"
 
-
align="right">4.65E+09</td>
 
-
      <td></td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS2 +
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">99</td>
 
-
      <td x:num="4950000000" x:fmla="=(10^B26)*C26*50"
 
-
align="right">4.95E+09</td>
 
-
      <td x:num="104.21052631578947" x:fmla="=(D26/D27)*100"
 
-
align="right">104.2105263</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS2 -
 
-
chlor</td>
 
-
      <td x:num="" align="right">6</td>
 
-
      <td x:num="" align="right">95</td>
 
-
      <td x:num="4750000000" x:fmla="=(10^B27)*C27*50"
 
-
align="right">4.75E+09</td>
 
-
      <td></td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td class="xl65" colspan="2"
 
-
style="height: 15pt; text-decoration: underline;" height="20">100
 
-
generations</td>
 
-
      <td></td>
 
-
      <td></td>
 
-
      <td></td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS1 +
 
-
chlor</td>
 
-
      <td x:num="" align="right">5</td>
 
-
      <td x:num="" align="right">130</td>
 
-
      <td x:num="650000000" x:fmla="=(10^B29)*C29*50"
 
-
align="right">650000000</td>
 
-
      <td x:num="" x:fmla="=(D29/D30)*100"
 
-
align="right">125</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS1 -
 
-
chlor</td>
 
-
      <td x:num="" align="right">5</td>
 
-
      <td x:num="" align="right">104</td>
 
-
      <td x:num="" x:fmla="=(10^B30)*C30*50"
 
-
align="right">520000000</td>
 
-
      <td></td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS2 +
 
-
chlor</td>
 
-
      <td x:num="" align="right">5</td>
 
-
      <td x:num="" align="right">124</td>
 
-
      <td x:num="620000000" x:fmla="=(10^B31)*C31*50"
 
-
align="right">620000000</td>
 
-
      <td x:num="118.0952380952381" x:fmla="=(D31/D32)*100"
 
-
align="right">118.0952381</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt;" height="20">PS2 -
 
-
chlor</td>
 
-
      <td x:num="" align="right">5</td>
 
-
      <td x:num="" align="right">105</td>
 
-
      <td x:num="" x:fmla="=(10^B32)*C32*50"
 
-
align="right">525000000</td>
 
-
      <td></td>
 
-
    </tr>
 
-
  </tbody>
 
-
</table>
 
-
</body>
 
-
</html>
 
-
<br><br>
 
-
 
-
<html>
 
-
<head>
 
-
  <meta content="text/html; charset=ISO-8859-1"
 
-
http-equiv="content-type">
 
-
  <title></title>
 
-
</head>
 
-
<body>
 
-
<table x:str=""
 
-
style="border-collapse: collapse; width: 507px;" border="0"
 
-
cellpadding="0" cellspacing="0" width="516">
 
-
  <col style="width: 110pt;" width="147"> <col
 
-
span="2" style="width: 48pt;" width="64"> <col
 
-
style="width: 85pt;" width="113"> <col
 
-
style="width: 96pt;" width="128"> <tbody>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt; font-weight: bold; width: 91px;"
 
-
height="20">Number of generations</td>
 
-
      <td style="font-weight: bold; width: 102px;">PS1</td>
 
-
      <td style="font-weight: bold; width: 102px;">PS2</td>
 
-
      <td style="font-weight: bold; width: 102px;">PS
 
-
(average)</td>
 
-
      <td style="font-weight: bold; width: 102px;">Standard
 
-
deviations</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt; width: 91px;" x:num=""
 
-
align="right" height="20">0</td>
 
-
      <td style="width: 102px;" x:num="" align="right">100</td>
 
-
      <td style="width: 102px;" x:num="" align="right">100</td>
 
-
      <td style="width: 102px;" x:num=""
 
-
x:fmla="=(B2+C2)/2" align="right">100</td>
 
-
      <td style="width: 102px;" x:num=""
 
-
x:fmla="=STDEV(B2,C2)" align="right">0</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt; width: 91px;" x:num=""
 
-
align="right" height="20">20</td>
 
-
      <td style="width: 102px;" x:num="59.245283018867923"
 
-
align="right">59.24528</td>
 
-
      <td style="width: 102px;" x:num="63.414634146341463"
 
-
align="right">63.41463</td>
 
-
      <td style="width: 102px;" x:num="61.329958582604689"
 
-
x:fmla="=(B3+C3)/2" align="right">61.32995858</td>
 
-
      <td style="width: 102px;" x:num="2.948176455384516"
 
-
x:fmla="=STDEV(B3,C3)" align="right">2.948176455</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt; width: 91px;" x:num=""
 
-
align="right" height="20">40</td>
 
-
      <td style="width: 102px;" x:num="95.973154362416096"
 
-
align="right">95.97315</td>
 
-
      <td style="width: 102px;" x:num="92.817679558011051"
 
-
align="right">92.81768</td>
 
-
      <td style="width: 102px;" x:num="94.395416960213566"
 
-
x:fmla="=(B4+C4)/2" align="right">94.39541696</td>
 
-
      <td style="width: 102px;" x:num="2.2312576320582482"
 
-
x:fmla="=STDEV(B4,C4)" align="right">2.231257632</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt; width: 91px;" x:num=""
 
-
align="right" height="20">60</td>
 
-
      <td style="width: 102px;" x:num="89.634146341463421"
 
-
align="right">89.63415</td>
 
-
      <td style="width: 102px;" x:num="104.6875"
 
-
align="right">104.6875</td>
 
-
      <td style="width: 102px;" x:num="97.160823170731703"
 
-
x:fmla="=(B5+C5)/2" align="right">97.16082317</td>
 
-
      <td style="width: 102px;" x:num="10.644328451550683"
 
-
x:fmla="=STDEV(B5,C5)" align="right">10.64432845</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt; width: 91px;" x:num=""
 
-
align="right" height="20">80</td>
 
-
      <td style="width: 102px;" x:num="136.55913978494624"
 
-
align="right">136.5591</td>
 
-
      <td style="width: 102px;" x:num="104.21052631578947"
 
-
align="right">104.2105</td>
 
-
      <td style="width: 102px;" x:num="120.38483305036786"
 
-
x:fmla="=(B6+C6)/2" align="right">120.3848331</td>
 
-
      <td style="width: 102px;" x:num="22.873923946023144"
 
-
x:fmla="=STDEV(B6,C6)" align="right">22.87392395</td>
 
-
    </tr>
 
-
    <tr style="height: 15pt;" height="20">
 
-
      <td style="height: 15pt; width: 91px;" x:num=""
 
-
align="right" height="20">100</td>
 
-
      <td style="width: 102px;" x:num="" align="right">125</td>
 
-
      <td style="width: 102px;" x:num="118.0952380952381"
 
-
align="right">118.0952</td>
 
-
      <td style="width: 102px;" x:num="121.54761904761905"
 
-
x:fmla="=(B7+C7)/2" align="right">121.547619</td>
 
-
      <td style="width: 102px;" x:num="4.8824039653356079"
 
-
x:fmla="=STDEV(B7,C7)" align="right">4.882403965</td>
 
-
    </tr>
 
-
  </tbody>
 
-
</table>
 
-
</body>
 
-
</html>
 
== Retinal ==
== Retinal ==

Revision as of 20:14, 24 October 2010