Team:SDU-Denmark/project-r

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(UV-Vis spectrophotometer determination of beta-carotene and retinal production)
(UV-Vis spectrophotometer determination of beta-carotene and retinal production)
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[[Image: Team-SDU-denmark-Retinal standarts.png |350px|]]
[[Image: Team-SDU-denmark-Retinal standarts.png |350px|]]
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The spectrum for the Beta-carotene looks normal and reliable, the standards for retinal however doesn’t look reliable. The spectrum for the two lowest concentrations appears to be the measurement jumps rapidly from nearly nothing to the maximum absorbance, this might be because the concentration of retinal is to low to be measured as a spectrum, but the concentration of the individual derivates of retinal might be high enough to be measured.<br>
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The spectrum for the Beta-carotene looks normal and reliable, the standards for retinal however doesn’t look reliable. The spectrum for the two lowest concentrations appears to be give a wrong picture from the measurement.<br>
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the measurement jumps rapidly from nearly nothing to the maximum absorbance, this might be because the concentration of retinal is to low to be measured as a spectrum, but the concentration of the individual derivates of retinal might be high enough to be measured.<br>
It might also just be that the concentrations are to low and something ells are influencing the measurements.<br>
It might also just be that the concentrations are to low and something ells are influencing the measurements.<br>
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Because of the error in the UV-vis spectre concerning retinal detection in both the standards and samples the next characterization experiments is preformed on a HPLC.<br><br></p>     
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Because of the error in the UV-vis spectre concerning retinal detection in both the standards and samples the next characterization experiments is preformed on a HPLC.
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Revision as of 14:26, 24 October 2010