Team:SDU-Denmark/project-r

From 2010.igem.org

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(Motility assay)
(Motility assay)
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The upper left picture is of ''E. coli'' strain '''DH5alpha''' that does not express flagella and therefor movement in the media should be minimal. The upper right picture is of the wild type ''E. coli'' strain '''MG1655''', this strain has abour 8-10 flagella per cell. These cells are expected to move farther than the DH5alpha. The lower left picture number three is of ''E. Coli'' strain '''MG1655 with pSB1C3-K343004'''. The lover right picture is of ''E. coli'' strain '''MG1655 with pSB3K3-K343004'''.<br>
The upper left picture is of ''E. coli'' strain '''DH5alpha''' that does not express flagella and therefor movement in the media should be minimal. The upper right picture is of the wild type ''E. coli'' strain '''MG1655''', this strain has abour 8-10 flagella per cell. These cells are expected to move farther than the DH5alpha. The lower left picture number three is of ''E. Coli'' strain '''MG1655 with pSB1C3-K343004'''. The lover right picture is of ''E. coli'' strain '''MG1655 with pSB3K3-K343004'''.<br>
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pSB1C3 is a high copy plasmid while pSB3K3 is a low-medium copy plasmid. The pictures show that the cells with pSB3k3-K343004 moves farther than cells containing pSB1C3-K343004. The promoters in K343004 is a constitutive promoter (tetR repressable promoter), bacterias containing a high copy plasmid with a constitutive promoter is forced to  This indicates a lower
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The two buttom pictures show that bacteria with pSB1C3-K343004 have not moved as far as the bacteria containing pSB3K3-K343004. pSB1C3 is a high copy plasmid while pSB3K3 is a low-medium copy plasmid. The promoters in K343004 is a constitutive promoter (tetR repressable promoter), bacteria containing a high copy plasmid with a constitutive promoter are more metabolically challanged than bacteria containing a low or medium copy plasmid with a constitutive promoter. The high copy bacteria.
Plates one and two did not contain antibiotics, and therefore contamination colonies are seen.<br><br>
Plates one and two did not contain antibiotics, and therefore contamination colonies are seen.<br><br>

Revision as of 16:50, 23 October 2010