Team:SDU-Denmark/project-r

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(Motility assay)
(Motility assay)
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===Motility assay===
===Motility assay===
<br>Exp. 1<br>
<br>Exp. 1<br>
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The purpose of this experiment is to see if the cells containing pSB1C3-K343004 move farther than the wild type cells. This would be an indication of hyperflagellation.<br><br>
 
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For the motility experiments we added 5ul of an ON culture to petridishes containing motility agar (LB media with 0.3% agar) instead of regular LA (Luria agar). This semifluid media lets the bacteria swimm more easily. <br>
 
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The plates were left in an incubator (37 degrees) for 24 hours.<br><br>
 
[[Image:Team-SDU-Denmark-Flagellamotility-exp1-DH5a.JPG|250px|thumb|left|DH5alpha]]
[[Image:Team-SDU-Denmark-Flagellamotility-exp1-DH5a.JPG|250px|thumb|left|DH5alpha]]
[[Image:Team-SDU-Denmark-Flagellamotility-exp1-MG1655.JPG|250px|thumb|left|MG1655]]
[[Image:Team-SDU-Denmark-Flagellamotility-exp1-MG1655.JPG|250px|thumb|left|MG1655]]
[[Image:Team-SDU-Denmark-Flagellamotility-exp1-FlhDCmutCP_i_pSB1C3_(LA+chlor).JPG|250px||thumb|left|pSB1C3-FlhDCmutCP(LA+chlor).]]
[[Image:Team-SDU-Denmark-Flagellamotility-exp1-FlhDCmutCP_i_pSB1C3_(LA+chlor).JPG|250px||thumb|left|pSB1C3-FlhDCmutCP(LA+chlor).]]
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[[Image:Team-SDU-Denmark-Flagellamotility-exp1-FlhDCmutCP_i_pSB3K3_(LA+kan).JPG|250px|thumb|left|pSB3K3-FlhDCmutCP(LA+Kan)]]<br><br>
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[[Image:Team-SDU-Denmark-Flagellamotility-exp1-FlhDCmutCP_i_pSB3K3_(LA+kan).JPG|250px|thumb|left|pSB3K3-FlhDCmutCP(LA+Kan)]]<br><br>The purpose of this experiment is to see if the cells containing pSB1C3-K343004 move farther than the wild type cells. This would be an indication of hyperflagellation.<br><br>
 +
For the motility experiments we added 5ul of an ON culture to petridishes containing motility agar (LB media with 0.3% agar) instead of regular LA (Luria agar). This semifluid media lets the bacteria swimm more easily. <br>
 +
The plates were left in an incubator (37 degrees) for 24 hours.<br><br>
 +
 
The picture number one to the left is of ''E. coli'' strain '''DH5alpha''' that does not express flagella and therefor movement in the media should be minimal. Picture number two is of the wild type ''E. coli'' strain '''MG1655''', this strain has abour 8-10 flagella per cell. These cells are expected to move farther than the DH5alpha. Picture number three is of ''E. Coli'' strain '''MG1655 with pSB1C3-K343004'''. Picture number four is of ''E. coli'' strain '''MG1655 with pSB3K3-K343004'''.<br>
The picture number one to the left is of ''E. coli'' strain '''DH5alpha''' that does not express flagella and therefor movement in the media should be minimal. Picture number two is of the wild type ''E. coli'' strain '''MG1655''', this strain has abour 8-10 flagella per cell. These cells are expected to move farther than the DH5alpha. Picture number three is of ''E. Coli'' strain '''MG1655 with pSB1C3-K343004'''. Picture number four is of ''E. coli'' strain '''MG1655 with pSB3K3-K343004'''.<br>
pSB1C3 is a high copy plasmid while pSB3K3 is a low-medium copy plasmid. The pictures show that the cells with pSB3k3-K343004 moves farther than cells containing pSB1C3-K343004. The promoters in K343004 is a constitutive promoter (tetR repressable promoter),  bacterias containing a high copy plasmid with a constitutive promoter is forced to  This indicates a lower  
pSB1C3 is a high copy plasmid while pSB3K3 is a low-medium copy plasmid. The pictures show that the cells with pSB3k3-K343004 moves farther than cells containing pSB1C3-K343004. The promoters in K343004 is a constitutive promoter (tetR repressable promoter),  bacterias containing a high copy plasmid with a constitutive promoter is forced to  This indicates a lower  

Revision as of 16:17, 23 October 2010