Team:SDU-Denmark/project-p

From 2010.igem.org

(Difference between revisions)
(Characterization)
(Computerized analysis of the bacterial motility with the THOR prototype by Unisensor A/S and the Unify software)
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This might indicate a decreased tumbling frequency, but the low motility of the bacteria, the inadequacy of the cell track software and the small amount of data analysed make results of this experiment unreliable and a new protocol had to be devised.<br><br>
This might indicate a decreased tumbling frequency, but the low motility of the bacteria, the inadequacy of the cell track software and the small amount of data analysed make results of this experiment unreliable and a new protocol had to be devised.<br><br>
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===Computerized analysis of the bacterial motility with the THOR prototype by [http://unisensor.dk/ Unisensor A/S] and the Unify software===
 
===Computerized analysis of the bacterial motility with the THOR prototype by [http://unisensor.dk/ Unisensor A/S] and the Unify software===
===Computerized analysis of the bacterial motility with the THOR prototype by [http://unisensor.dk/ Unisensor A/S] and the Unify software===
For this experiment we changed our protocol for cultivating swimming bacteria in order to optimize their mobility [http://2010.igem.org/Team:SDU-Denmark/protocols#PS1.2 PS1.2]<br>
For this experiment we changed our protocol for cultivating swimming bacteria in order to optimize their mobility [http://2010.igem.org/Team:SDU-Denmark/protocols#PS1.2 PS1.2]<br>
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From this file, a trackplot was generated, showing each bacteria's path over the recorded time:<br>
From this file, a trackplot was generated, showing each bacteria's path over the recorded time:<br>
[[Image:TrackPlotTagged.png|thumb|400px|center|Bacteria containing K343007 tracked over 7.5 seconds]]<br>
[[Image:TrackPlotTagged.png|thumb|400px|center|Bacteria containing K343007 tracked over 7.5 seconds]]<br>
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We started out with extracting the mean velocity in um/frame for the photosensor and wildtype bacteria when exposed red and blue light, the eman was extracted from around 100 paths for each of the 4 different samples. What we hoped to see was that bacteria that tumble less would have a higher mean velocity, than bacteria that exhibit a normal rate of tumbling (the wildtype):<br>
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We started out with extracting the mean velocity in um/frame for the photosensor and wildtype bacteria when exposed red and blue light, the mean was extracted from around 100 paths for each of the 4 different samples (only taken from the experiment where we shifted between red and blue light, data from the light intensity and gradient experiment were not included.). What we hoped to see was that bacteria that tumble less would have a higher mean velocity, than bacteria that exhibit a normal rate of tumbling (the wildtype):<br>
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[[Image:Red_blue_PS_noPS.jpg|center]]
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Revision as of 16:49, 27 October 2010