Team:SDU-Denmark/project-p

From 2010.igem.org

(Difference between revisions)
(Characterization)
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This might indicate a decreased tumbling frequency, but the low motility of the bacteria, the inadequacy of the cell track software and the small amount of data analysed make results of this experiment unreliable and a new protocol had to be devised.<br><br>
This might indicate a decreased tumbling frequency, but the low motility of the bacteria, the inadequacy of the cell track software and the small amount of data analysed make results of this experiment unreliable and a new protocol had to be devised.<br><br>
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===Computerized analysis of the bacterial motility with the THOR prototype by [http://unisensor.dk/ Unisensor A/S] and the Unify software===
===Computerized analysis of the bacterial motility with the THOR prototype by [http://unisensor.dk/ Unisensor A/S] and the Unify software===
===Computerized analysis of the bacterial motility with the THOR prototype by [http://unisensor.dk/ Unisensor A/S] and the Unify software===
For this experiment we changed our protocol for cultivating swimming bacteria in order to optimize their mobility [http://2010.igem.org/Team:SDU-Denmark/protocols#PS1.2 PS1.2]<br>
For this experiment we changed our protocol for cultivating swimming bacteria in order to optimize their mobility [http://2010.igem.org/Team:SDU-Denmark/protocols#PS1.2 PS1.2]<br>
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Here is an example of a .txt file containing the results: [http://2010.igem.org/wiki/images/6/6b/TrackResult_iGEM-WTred1.txt Tracks of wildtype bacteria exposed to red light]. If interested, please request the whole dataset from us, which will be sent out as a 450mb zip file.<br>
Here is an example of a .txt file containing the results: [http://2010.igem.org/wiki/images/6/6b/TrackResult_iGEM-WTred1.txt Tracks of wildtype bacteria exposed to red light]. If interested, please request the whole dataset from us, which will be sent out as a 450mb zip file.<br>
From this file, a trackplot was generated, showing each bacteria's path over the recorded time:<br>
From this file, a trackplot was generated, showing each bacteria's path over the recorded time:<br>
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[[Image:TrackPlotTagged.png|thumb|center|Bacteria containing K343007 tracked over 7.5 seconds]]
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[[Image:TrackPlotTagged.png|thumb|400px|center|Bacteria containing K343007 tracked over 7.5 seconds]]<br>
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We started out with extracting the mean velocity in um/frame for the photosensor and wildtype bacteria when exposed red and blue light, the eman was extracted from around 100 paths for each of the 4 different samples. What we hoped to see was that bacteria that tumble less would have a higher mean velocity, than bacteria that exhibit a normal rate of tumbling (the wildtype):<br>
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Revision as of 16:42, 27 October 2010