Team:SDU-Denmark/project-p

From 2010.igem.org

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(K343007)
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From the results of the first experiment we expected the culture containing BBa_K343007 to spread out in the dark and not to spread when exposed to light. The wildtype bacteria should spread out evenly no matter if exposed to light or not and the non-motile strain (DH5alpha) should not move regardless of the light conditions. Our expectations from the first experiment were fulfilled, as the bacteria behaved exactly as expected. This made it possible to conclude that the photosensor has an effect on the bacteria's tumbling frequency, but if it does in fact reduce the tumbling is not possible to say, since both an increased and reduced tumbling frequency will look alike.<br>
From the results of the first experiment we expected the culture containing BBa_K343007 to spread out in the dark and not to spread when exposed to light. The wildtype bacteria should spread out evenly no matter if exposed to light or not and the non-motile strain (DH5alpha) should not move regardless of the light conditions. Our expectations from the first experiment were fulfilled, as the bacteria behaved exactly as expected. This made it possible to conclude that the photosensor has an effect on the bacteria's tumbling frequency, but if it does in fact reduce the tumbling is not possible to say, since both an increased and reduced tumbling frequency will look alike.<br>
After this we could conclude that the part had an impact on the bacterial motility, but we had to find out which. This lead us to our next experiment, which was intended for determining what happened to the tumbling frequency:<br>
After this we could conclude that the part had an impact on the bacterial motility, but we had to find out which. This lead us to our next experiment, which was intended for determining what happened to the tumbling frequency:<br>
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<br>[[Image:Team-SDU-Denmark-MG1655.JPG|210px|MG1655]] [[Image:Team-SDU-Denmark-Photosensor.JPG|210px|Photosensor]] [[Image:Team-SDU-Denmark-DH5alpha.JPG|210px|DH5alpha]]
'''3. Videomicroscopy and computer analysis:'''<br>
'''3. Videomicroscopy and computer analysis:'''<br>
To get an exact idea of what is happening to the bacteria when expressing the photosensor we decided on doing video microscopy of motile bacteria. The bacterial cultures were prepared acording to protocol (insert link). Wildtype MG1655 and DH5alpha was used as controls.  
To get an exact idea of what is happening to the bacteria when expressing the photosensor we decided on doing video microscopy of motile bacteria. The bacterial cultures were prepared acording to protocol (insert link). Wildtype MG1655 and DH5alpha was used as controls.  

Revision as of 14:53, 24 October 2010