Team:SDU-Denmark/project-p

From 2010.igem.org

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(Computerized analysis of the bacterial motility with the THOR prototype by Unisensor A/S and the Unify software)
(Phase contrast of ON cultures)
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The purpose of the phase contrast microscopy is to see if the transformants are visibly different from the wild type. Since the FlhDC operon is not only an important part of the flagella synthesis but is also coupled to cell metabolism [[http://2010.igem.org/Team:SDU-Denmark/project-p#References 3]], it might be possible to see difference in morphology caused by an alterations in the cell cycle. <br>
The purpose of the phase contrast microscopy is to see if the transformants are visibly different from the wild type. Since the FlhDC operon is not only an important part of the flagella synthesis but is also coupled to cell metabolism [[http://2010.igem.org/Team:SDU-Denmark/project-p#References 3]], it might be possible to see difference in morphology caused by an alterations in the cell cycle. <br>
Phase contrast pictures were taken of wild type MG1655, MG1655/pSB1C3-K343004 and MG1655/pSB3K3-K343004.<br><br>
Phase contrast pictures were taken of wild type MG1655, MG1655/pSB1C3-K343004 and MG1655/pSB3K3-K343004.<br><br>
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[[Image: Team SDU-Denmark Phase contrast MG1655.JPG|210px| Phase contrast of MG1655]]
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[[Image:Team-SDU-Denmark-Flagella-PCM-1.jpg|630px|center|thumb]]<br><br>
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[[Image: Team SDU-Denmark Phase contrast pSB1C3.JPG|210px| Phase contrast of MG1655/pSB1C3-K343004]]
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[[Image: Team SDU-Denmark Phase contrast pSB3K3.JPG|210px| Phase contrast of MG1655/pSB3K3-K343004]]<br><br>
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The pictures clearly show a difference in morphology of the wild type (picture 1) and the two transformants (picture 2-pSB1C3 and 3-pSB3K3). The wild type cells are small cells, while the transformants are obviously larger, which indicates a lower metabolism of the transformants. Cells with impaired metabolism divide slower and are larger than cells with normal metabolism (wild type), since it takes the impaired cells longer to reach the mitotic phase of the cell cycle.
The pictures clearly show a difference in morphology of the wild type (picture 1) and the two transformants (picture 2-pSB1C3 and 3-pSB3K3). The wild type cells are small cells, while the transformants are obviously larger, which indicates a lower metabolism of the transformants. Cells with impaired metabolism divide slower and are larger than cells with normal metabolism (wild type), since it takes the impaired cells longer to reach the mitotic phase of the cell cycle.

Revision as of 18:28, 27 October 2010