Team:SDU-Denmark/project-i
From 2010.igem.org
The Idea
Inspired by an article on flow generated in a micro-capillary tube by a bacterial "pump" [1] we have decided to attempt construction of a similar system. Our approach centers on E. coli that will be modified to hyper-express flagella, in an attempt to increase force generation potential. These bacteria will be attached to a surface, and their flagella should therefor generate flow in the surrounding liquid.
Regulation of the pump will be introduced through a photo-sensing chimeric fusion protein that has recently been shown to integrate with the E. coli chemotaxis system [2], to control tumbling frequencies in our bacteria, thereby giving us control over the amount of turbulence disrupting our pump via a blue light source. The parts contributed will in effect confer phototaxic ability to E. coli.
We suggest that our system will also be convertible to a mixing device, for mixing fluids in micro-capillaries, and other related environments.
Both sub-project will contribute new parts to the registry.
--CKurtzhals 21:39, 1 July 2010 (UTC)