"
Team:SDU-Denmark/primers
From 2010.igem.org
Revision as of 13:31, 21 October 2010 by MarieBrønsNørgaard (Talk | contribs)
Primers
On this page we list all the primers we designed and used during the summer. All primers were kindly provided by DNA Technology.
<!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01//EN" "http://www.w3.org/TR/html4/strict.dtd">
| Primer name | Sequence | Tm (C) |
| FlhDC fw | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAA-3' | 88.3 |
| FlhDC rv | 5'-GTTTCTTCCTGCAGCGGCCGCTACTATTAAACAGCCTGTACTCTCTGTTC-3' | 88.3 |
| FlhDC mutation fw* | 5'-TTGGCAGCTTTGCCCGCAGCTTATG-3' | 61.0 |
| FlhDC mutation rv* | 5'-CATAAGCTGCGGGCAAAGCTGCCAA-3' | 61.0 |
| Photosensor fw | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGTGGGACTTACGACCTC-3' | 73.1 |
| Photosensor rv | 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGAAGGTTTCCCAGTTCGCATC-3' | 73.9 |
| NinaB fw | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGCAGCCGGTGTCTTCAAGAG-3' | 73.1 |
| NinaB rv | 5'-GTGTCTTCCTGCAGCGGCCGCTACTAGTACTAAATGGCATTGGGTGCAAACCATC-3' | 72.4 |
| NinaB 2 fw** | 5'-CCGCTTCTAGATGGCAGCCGGTGTCTTCAAGAG-3' | 68.1 |
| NinaB 2 rv** | 5'-CGCTACTAGTACTAAATGGCATTGGGTGCAAACCATC-3' | 65.6 |
(*) Mutation primers were designed to introduce a silent mutation at position 822 in the operon to eliminate an illegal PstI site.
(**) Due to the formation of hairpin structures of primers ninaB fw and ninaB rv, new primers were designed containing only the innermost restriction sites (XbaI and SpeI).
Insert clever content here.
