"
Team:SDU-Denmark/primers
From 2010.igem.org
(Difference between revisions)
m (→Primers) |
m (→Primers) |
||
| Line 27: | Line 27: | ||
<tr> | <tr> | ||
<td | <td | ||
| - | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 60px;">Primer |
name</td> | name</td> | ||
<td | <td | ||
| - | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 638px;">Sequence</td> |
<td | <td | ||
style="width: 51px; font-family: Helvetica,Arial,sans-serif; height: 44px;">Tm | style="width: 51px; font-family: Helvetica,Arial,sans-serif; height: 44px;">Tm | ||
| Line 37: | Line 37: | ||
<tr> | <tr> | ||
<td | <td | ||
| - | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 60px;">FlhDC |
fw</td> | fw</td> | ||
<td | <td | ||
| - | style="font-family: Courier New; height: 44px; width: | + | style="font-family: Courier New; height: 44px; width: 638px;">5'-GTTTCTTC<span |
style="color: rgb(80, 204, 56);">GAATTC</span>GCGGCCGCT<span | style="color: rgb(80, 204, 56);">GAATTC</span>GCGGCCGCT<span | ||
style="color: rgb(80, 204, 56);">TCTAG</span><span | style="color: rgb(80, 204, 56);">TCTAG</span><span | ||
| Line 50: | Line 50: | ||
<tr> | <tr> | ||
<td | <td | ||
| - | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 60px;">FlhDC |
rv</td> | rv</td> | ||
<td | <td | ||
| - | style="font-family: Courier New; height: 44px; width: | + | style="font-family: Courier New; height: 44px; width: 638px;">5'-GTTTCTTC<span |
style="color: rgb(80, 204, 56);">CTGCAG</span>CGGCCGCTACTATT<span | style="color: rgb(80, 204, 56);">CTGCAG</span>CGGCCGCTACTATT<span | ||
style="color: rgb(88, 115, 248);">AAACAGCCTGTACTCTCTGTTC</span>-3'</td> | style="color: rgb(88, 115, 248);">AAACAGCCTGTACTCTCTGTTC</span>-3'</td> | ||
| Line 61: | Line 61: | ||
<tr> | <tr> | ||
<td | <td | ||
| - | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 60px;">FlhDC |
mutation fw*</td> | mutation fw*</td> | ||
<td | <td | ||
| - | style="font-family: Courier New; height: 44px; width: | + | style="font-family: Courier New; height: 44px; width: 638px;">5'-<span |
style="color: rgb(88, 115, 248);">TTGGCAGCTTTGCC<span | style="color: rgb(88, 115, 248);">TTGGCAGCTTTGCC<span | ||
style="color: rgb(204, 51, 204);">C</span>GCAGCTTATG</span>-3'</td> | style="color: rgb(204, 51, 204);">C</span>GCAGCTTATG</span>-3'</td> | ||
| Line 72: | Line 72: | ||
<tr> | <tr> | ||
<td | <td | ||
| - | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 60px;">FlhDC |
mutation rv*</td> | mutation rv*</td> | ||
<td | <td | ||
| - | style="font-family: Courier New; height: 44px; width: | + | style="font-family: Courier New; height: 44px; width: 638px;">5'-<span |
style="color: rgb(88, 115, 248);">CATAAGCTGC<span | style="color: rgb(88, 115, 248);">CATAAGCTGC<span | ||
style="color: rgb(204, 51, 204);">G</span>GGCAAAGCTGCCAA</span>-3'</td> | style="color: rgb(204, 51, 204);">G</span>GGCAAAGCTGCCAA</span>-3'</td> | ||
| Line 83: | Line 83: | ||
<tr> | <tr> | ||
<td | <td | ||
| - | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 60px;">Photosensor |
fw</td> | fw</td> | ||
<td | <td | ||
| - | style="font-family: Courier New; height: 44px; width: | + | style="font-family: Courier New; height: 44px; width: 638px;">5'-GTTTCTTC<span |
style="color: rgb(80, 204, 56);">GAATTC</span>GCGGCCGCT<span | style="color: rgb(80, 204, 56);">GAATTC</span>GCGGCCGCT<span | ||
style="color: rgb(80, 204, 56);">TCTAG</span><span | style="color: rgb(80, 204, 56);">TCTAG</span><span | ||
| Line 96: | Line 96: | ||
<tr> | <tr> | ||
<td | <td | ||
| - | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 60px;">Photosensor |
rv</td> | rv</td> | ||
<td | <td | ||
| - | style="font-family: Courier New; height: 44px; width: | + | style="font-family: Courier New; height: 44px; width: 638px;">5'-GTTTCTTC<span |
style="color: rgb(80, 204, 56);">CTGCAG</span>CGGCCGCT<span | style="color: rgb(80, 204, 56);">CTGCAG</span>CGGCCGCT<span | ||
style="color: rgb(80, 204, 56);">ACTAGT</span>A<span | style="color: rgb(80, 204, 56);">ACTAGT</span>A<span | ||
| Line 108: | Line 108: | ||
<tr> | <tr> | ||
<td | <td | ||
| - | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 60px;">NinaB |
fw</td> | fw</td> | ||
<td | <td | ||
| - | style="font-family: Courier New; height: 44px; width: | + | style="font-family: Courier New; height: 44px; width: 638px;">5'-<span |
style="color: rgb(80, 204, 56);"><span | style="color: rgb(80, 204, 56);"><span | ||
style="color: black;">GTTTCTTC</span>GAATTC</span>GCGGCCGCT<span | style="color: black;">GTTTCTTC</span>GAATTC</span>GCGGCCGCT<span | ||
| Line 122: | Line 122: | ||
<tr> | <tr> | ||
<td | <td | ||
| - | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 60px;">NinaB |
rv</td> | rv</td> | ||
<td | <td | ||
| - | style="font-family: Courier New; height: 44px; width: | + | style="font-family: Courier New; height: 44px; width: 638px;">5'-<span |
style="color: rgb(80, 204, 56);"><span | style="color: rgb(80, 204, 56);"><span | ||
style="color: black;">GTGTCTTC</span>CTGCAG</span>CGGCCGCT<span | style="color: black;">GTGTCTTC</span>CTGCAG</span>CGGCCGCT<span | ||
| Line 135: | Line 135: | ||
<tr> | <tr> | ||
<td | <td | ||
| - | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 60px;">NinaB |
2 fw**</td> | 2 fw**</td> | ||
<td | <td | ||
| - | style="font-family: Courier New; height: 44px; width: | + | style="font-family: Courier New; height: 44px; width: 638px;">5'-CCGCT<span |
style="color: rgb(80, 204, 56);">TCTAG</span><span | style="color: rgb(80, 204, 56);">TCTAG</span><span | ||
style="color: rgb(88, 115, 248);"><span | style="color: rgb(88, 115, 248);"><span | ||
| Line 147: | Line 147: | ||
<tr> | <tr> | ||
<td | <td | ||
| - | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 60px;">NinaB |
2 rv**</td> | 2 rv**</td> | ||
<td | <td | ||
| - | style="font-family: Courier New; height: 44px; width: | + | style="font-family: Courier New; height: 44px; width: 638px;">5'-CGCT<span |
style="color: rgb(80, 204, 56);">ACTAGT</span>A<span | style="color: rgb(80, 204, 56);">ACTAGT</span>A<span | ||
style="color: rgb(88, 115, 248);">CTAAATGGCATTGGGTGCAAACCATC</span>-3'</td> | style="color: rgb(88, 115, 248);">CTAAATGGCATTGGGTGCAAACCATC</span>-3'</td> | ||
Revision as of 14:38, 21 October 2010
Primers
On this page we list all the primers we designed and used during the summer. All primers were kindly provided by DNA Technology.
In the following, coding sequences are colored blue, restriction sites are colored green and nucleotides that are both are shown in orange.
The mutation sites in the FlhDC mutation primers are shown in purple.
| Primer name | Sequence | Tm (C) |
| FlhDC fw | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAA-3' | 88.3 |
| FlhDC rv | 5'-GTTTCTTCCTGCAGCGGCCGCTACTATTAAACAGCCTGTACTCTCTGTTC-3' | 88.3 |
| FlhDC mutation fw* | 5'-TTGGCAGCTTTGCCCGCAGCTTATG-3' | 61.0 |
| FlhDC mutation rv* | 5'-CATAAGCTGCGGGCAAAGCTGCCAA-3' | 61.0 |
| Photosensor fw | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGTGGGACTTACGACCTC-3' | 73.1 |
| Photosensor rv | 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGAAGGTTTCCCAGTTCGCATC-3' | 73.9 |
| NinaB fw | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGCAGCCGGTGTCTTCAAGAG-3' | 73.1 |
| NinaB rv | 5'-GTGTCTTCCTGCAGCGGCCGCTACTAGTACTAAATGGCATTGGGTGCAAACCATC-3' | 72.4 |
| NinaB 2 fw** | 5'-CCGCTTCTAGATGGCAGCCGGTGTCTTCAAGAG-3' | 68.1 |
| NinaB 2 rv** | 5'-CGCTACTAGTACTAAATGGCATTGGGTGCAAACCATC-3' | 65.6 |
(*) Mutation primers were designed to introduce a silent mutation at position 822 in the operon to eliminate an illegal PstI site.
(**) Due to the formation of hairpin structures of primers ninaB fw and ninaB rv, new primers were designed containing only the innermost restriction sites (XbaI and SpeI).
Everything you need to get the party started!
