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Team:SDU-Denmark/primers
From 2010.igem.org
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On this page we list all the primers we designed and used during the summer. All primers were kindly provided by [http://www.dna-technology.dk/ DNA Technology]. <br><br> | On this page we list all the primers we designed and used during the summer. All primers were kindly provided by [http://www.dna-technology.dk/ DNA Technology]. <br><br> | ||
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<html> | <html> | ||
<head> | <head> | ||
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<tr> | <tr> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 50px;">Primer |
name</td> | name</td> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 648px;">Sequence</td> |
<td | <td | ||
style="width: 51px; font-family: Helvetica,Arial,sans-serif; height: 44px;">Tm | style="width: 51px; font-family: Helvetica,Arial,sans-serif; height: 44px;">Tm | ||
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<tr> | <tr> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 50px;">FlhDC |
fw</td> | fw</td> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Courier New; height: 44px; width: 648px;">5'-GTTTCTTC<span |
style="color: rgb(80, 204, 56);">GAATTC</span>GCGGCCGCT<span | style="color: rgb(80, 204, 56);">GAATTC</span>GCGGCCGCT<span | ||
style="color: rgb(80, 204, 56);">TCTAG</span><span | style="color: rgb(80, 204, 56);">TCTAG</span><span | ||
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<tr> | <tr> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 50px;">FlhDC |
rv</td> | rv</td> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Courier New; height: 44px; width: 648px;">5'-GTTTCTTC<span |
style="color: rgb(80, 204, 56);">CTGCAG</span>CGGCCGCTACTATT<span | style="color: rgb(80, 204, 56);">CTGCAG</span>CGGCCGCTACTATT<span | ||
style="color: rgb(88, 115, 248);">AAACAGCCTGTACTCTCTGTTC</span>-3'</td> | style="color: rgb(88, 115, 248);">AAACAGCCTGTACTCTCTGTTC</span>-3'</td> | ||
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<tr> | <tr> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 50px;">FlhDC |
mutation fw*</td> | mutation fw*</td> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Courier New; height: 44px; width: 648px;">5'-<span |
style="color: rgb(88, 115, 248);">TTGGCAGCTTTGCCCGCAGCTTATG</span>-3'</td> | style="color: rgb(88, 115, 248);">TTGGCAGCTTTGCCCGCAGCTTATG</span>-3'</td> | ||
<td | <td | ||
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<tr> | <tr> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 50px;">FlhDC |
mutation rv*</td> | mutation rv*</td> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Courier New; height: 44px; width: 648px;">5'-<span |
style="color: rgb(88, 115, 248);">CATAAGCTGCGGGCAAAGCTGCCAA</span>-3'</td> | style="color: rgb(88, 115, 248);">CATAAGCTGCGGGCAAAGCTGCCAA</span>-3'</td> | ||
<td | <td | ||
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<tr> | <tr> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 50px;">Photosensor |
fw</td> | fw</td> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Courier New; height: 44px; width: 648px;">5'-GTTTCTTC<span |
style="color: rgb(80, 204, 56);">GAATTC</span>GCGGCCGCT<span | style="color: rgb(80, 204, 56);">GAATTC</span>GCGGCCGCT<span | ||
style="color: rgb(80, 204, 56);">TCTAG</span><span | style="color: rgb(80, 204, 56);">TCTAG</span><span | ||
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<tr> | <tr> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 50px;">Photosensor |
rv</td> | rv</td> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Courier New; height: 44px; width: 648px;">5'-GTTTCTTC<span |
style="color: rgb(80, 204, 56);">CTGCAG</span>CGGCCGCT<span | style="color: rgb(80, 204, 56);">CTGCAG</span>CGGCCGCT<span | ||
style="color: rgb(80, 204, 56);">ACTAGT</span>A<span | style="color: rgb(80, 204, 56);">ACTAGT</span>A<span | ||
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<tr> | <tr> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 50px;">NinaB |
fw</td> | fw</td> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Courier New; height: 44px; width: 648px;">5'-<span |
style="color: rgb(80, 204, 56);"><span | style="color: rgb(80, 204, 56);"><span | ||
style="color: black;">GTTTCTTC</span>GAATTC</span>GCGGCCGCT<span | style="color: black;">GTTTCTTC</span>GAATTC</span>GCGGCCGCT<span | ||
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<tr> | <tr> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 50px;">NinaB |
rv</td> | rv</td> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Courier New; height: 44px; width: 648px;">5'-<span |
style="color: rgb(80, 204, 56);"><span | style="color: rgb(80, 204, 56);"><span | ||
style="color: black;">GTGTCTTC</span>CTGCAG</span>CGGCCGCT<span | style="color: black;">GTGTCTTC</span>CTGCAG</span>CGGCCGCT<span | ||
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<tr> | <tr> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 50px;">NinaB |
2 fw**</td> | 2 fw**</td> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Courier New; height: 44px; width: 648px;">5'-CCGCT<span |
style="color: rgb(80, 204, 56);">TCTAG</span><span | style="color: rgb(80, 204, 56);">TCTAG</span><span | ||
style="color: rgb(88, 115, 248);"><span | style="color: rgb(88, 115, 248);"><span | ||
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<tr> | <tr> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Helvetica,Arial,sans-serif; height: 44px; width: 50px;">NinaB |
2 rv**</td> | 2 rv**</td> | ||
<td | <td | ||
| - | style=" | + | style="font-family: Courier New; height: 44px; width: 648px;">5'-CGCT<span |
style="color: rgb(80, 204, 56);">ACTAGT</span>A<span | style="color: rgb(80, 204, 56);">ACTAGT</span>A<span | ||
style="color: rgb(88, 115, 248);">CTAAATGGCATTGGGTGCAAACCATC</span>-3'</td> | style="color: rgb(88, 115, 248);">CTAAATGGCATTGGGTGCAAACCATC</span>-3'</td> | ||
Revision as of 13:43, 21 October 2010
Primers
On this page we list all the primers we designed and used during the summer. All primers were kindly provided by DNA Technology.
| Primer name | Sequence | Tm (C) |
| FlhDC fw | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAA-3' | 88.3 |
| FlhDC rv | 5'-GTTTCTTCCTGCAGCGGCCGCTACTATTAAACAGCCTGTACTCTCTGTTC-3' | 88.3 |
| FlhDC mutation fw* | 5'-TTGGCAGCTTTGCCCGCAGCTTATG-3' | 61.0 |
| FlhDC mutation rv* | 5'-CATAAGCTGCGGGCAAAGCTGCCAA-3' | 61.0 |
| Photosensor fw | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGTGGGACTTACGACCTC-3' | 73.1 |
| Photosensor rv | 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGAAGGTTTCCCAGTTCGCATC-3' | 73.9 |
| NinaB fw | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGCAGCCGGTGTCTTCAAGAG-3' | 73.1 |
| NinaB rv | 5'-GTGTCTTCCTGCAGCGGCCGCTACTAGTACTAAATGGCATTGGGTGCAAACCATC-3' | 72.4 |
| NinaB 2 fw** | 5'-CCGCTTCTAGATGGCAGCCGGTGTCTTCAAGAG-3' | 68.1 |
| NinaB 2 rv** | 5'-CGCTACTAGTACTAAATGGCATTGGGTGCAAACCATC-3' | 65.6 |
(*) Mutation primers were designed to introduce a silent mutation at position 822 in the operon to eliminate an illegal PstI site.
(**) Due to the formation of hairpin structures of primers ninaB fw and ninaB rv, new primers were designed containing only the innermost restriction sites (XbaI and SpeI).
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