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Team:SDU-Denmark/primers
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== '''Primers''' == | == '''Primers''' == | ||
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| - | On this page we list all the primers we designed and used during the summer.<br><br> | + | On this page we list all the primers we designed and used during the summer. All primers were kindly provided by DNA Technology. <br><br> |
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^ Mutation primers to introduce a silent mutation at position 822 in the operon<br> | ^ Mutation primers to introduce a silent mutation at position 822 in the operon<br> | ||
| - | ^^ Due to the formation of hairpin structures of beta-caroten monooxygenase primer 1, primers with prefix and suffix only containing the restrictionsites of | + | ^^ Due to the formation of hairpin structures of beta-caroten monooxygenase primer 1, primers with prefix and suffix only containing the restrictionsites of XbaI and SpeI respectively were designed.<br><br> |
Revision as of 18:57, 19 October 2010
Primers
On this page we list all the primers we designed and used during the summer. All primers were kindly provided by DNA Technology.
| Primer name | coding sequence | Tm (celcius) | sequence with prefix or suffix | Tm (celcius) |
| FlhD/C primer fw | 5'-ATGCATACCTCCGAGTTGCTGAAA-3' | 55.7 | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAA-3' | 88.3 |
| FlhD/C primer rv | 5'-AAACAGCCTGTACTCTCTGTTC-3' | 53 | 5'-GTTTCTTCCTGCAGCGGCCGCTACTATTAAACAGCCTGTACTCTCTGTTC-3' | 88.3 |
| FlhD/C mutation primer fw^ | 5'-TTGGCAGCTTTGCCCGCAGCTTATG-3' | 61 | ||
| FlhD/C mutation primer rv^ | 5'-CATAAGCTGCGGGCAAAGCTGCCAA-3' | 61 | ||
| Photosensor primer fw | 5'-ATGGTGGGACTTACGACCTC-3' | 53.8 | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGTGGGACTTACGACCTC-3' | 73.1 |
| Photosensor primer rv | 5'-TCAGAAGGTTTCCCAGTTCGCATC-3' | 57.4 | 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGAAGGTTTCCCAGTTCGCATC-3' | 73.9 |
| beta-caroten monooxygenase primer1 fw | 5'-ATGGCAGCCGGTGTCTTCAAGAG-3' | 58.8 | 5'-GAATTCGCGGCCGCTTCTAGATGGCAGCCGGTGTCTTCAAGAG-3' | 73.1 |
| beta-caroten monooxygenase primer1 rv | 5'-CTAAATGGCATTGGGTGCAAACCATC-3' | 58 | 5'-CTGCAGCGGCCGCTACTAGTACTAAATGGCATTGGGTGCAAACCATC-3' | 72.4 |
| beta-caroten monooxygenase primer2 fw^^ | 5'-ATGGCAGCCGGTGTCTTCAAGAG-3' | 58.8 | 5'-CCGCTTCTAGATGGCAGCCGGTGTCTTCAAGAG-3' | 68.1 |
| beta-caroten monooxygenase primer2 rv^^ | 5'-CTAAATGGCATTGGGTGCAAACCATC-3' | 58 | 5'-CGCTACTAGTACTAAATGGCATTGGGTGCAAACCATC-3' | 65.6 |
^ Mutation primers to introduce a silent mutation at position 822 in the operon
^^ Due to the formation of hairpin structures of beta-caroten monooxygenase primer 1, primers with prefix and suffix only containing the restrictionsites of XbaI and SpeI respectively were designed.
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