Team:SDU-Denmark/primers

From 2010.igem.org

(Difference between revisions)

Revision as of 17:49, 17 October 2010

Primers

On this page we list all the primers we designed and used during the summer.

Primer name	coding sequence	Tm (celcius)	sequence with prefix or suffix	Tm (celcius)
FlhD/C primer fw	5'-ATGCATACCTCCGAGTTGCTGAAA-3'	55.7	5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAA-3'	88.3
FlhD/C primer rv	5'-AAACAGCCTGTACTCTCTGTTC-3'	53	5'-GTTTCTTCCTGCAGCGGCCGCTACTATTAAACAGCCTGTACTCTCTGTTC-3'	88.3
FlhD/C mutation primer fw^	5'-TTGGCAGCTTTGCCCGCAGCTTATG-3'	61
FlhD/C mutation primer rv^	5'-CATAAGCTGCGGGCAAAGCTGCCAA-3'	61
Photosensor primer fw	5'-ATGGTGGGACTTACGACCTC-3'	53.8	5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGTGGGACTTACGACCTC-3'	73.1
Photosensor primer rv	5'-TCAGAAGGTTTCCCAGTTCGCATC-3'	57.4	5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGAAGGTTTCCCAGTTCGCATC-3'	73.9
beta-caroten monooxygenase primer1 fw	5'-ATGGCAGCCGGTGTCTTCAAGAG-3'	58.8	5'-GAATTCGCGGCCGCTTCTAGATGGCAGCCGGTGTCTTCAAGAG-3'	73.1
beta-caroten monooxygenase primer1 rv	5'-CTAAATGGCATTGGGTGCAAACCATC-3'	58	5'-CTGCAGCGGCCGCTACTAGTACTAAATGGCATTGGGTGCAAACCATC-3'	72.4
beta-caroten monooxygenase primer2 fw^^	5'-ATGGCAGCCGGTGTCTTCAAGAG-3'	58.8	5'-CCGCTTCTAGATGGCAGCCGGTGTCTTCAAGAG-3'	68.1
beta-caroten monooxygenase primer2 rv^^	5'-CTAAATGGCATTGGGTGCAAACCATC-3'	58	5'-CGCTACTAGTACTAAATGGCATTGGGTGCAAACCATC-3'	65.6

^ Mutation primers to introduce a silent mutation at position 822 in the operon
^^ Due to the formation of hairpin structures of beta-caroten monooxygenase primer 1, primers with prefix and suffix only containing the restrictionsites of xbaI and SpeI respectively were designed.

Insert clever content here.

@@ Line 8: / Line 8: @@
 <br>
 On this page we list all the primers we designed and used during the summer.<br><br>
-<table style="text-align: left; width: 100%;" border="1"
+<table style="text-align: left;" border="1"
   cellpadding="2" cellspacing="2">
      <tr>