Team:SDU-Denmark/notebook

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(Retinal Production)
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== Retinal Production ==
== Retinal Production ==
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* Decided to atempt to make our backteria synthesise retinal endogenously.  
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* Decided to atempt to make our bacteria synthesize retinal endogenously.  
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* Designed a new biobrick around the ninaB gene from D. melanogaster, that has been shown to produce a beta-carotene 15,15'-monooxygenase [http://www.jbc.org/content/275/16/11915.long][http://www.ncbi.nlm.nih.gov/pmc/articles/PMC14720/?tool=pubmed].  
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* Designed a new BioBrick around the ''ninaB'' gene from ''D. melanogaster'', that has been shown to produce a beta-carotene 15,15'-monooxygenase [http://www.jbc.org/content/275/16/11915.long][http://www.ncbi.nlm.nih.gov/pmc/articles/PMC14720/?tool=pubmed].  
* Isolated the gene sequence, and suggested biobricks for the coding region (K343001)
* Isolated the gene sequence, and suggested biobricks for the coding region (K343001)
* Proposed four biobricks
* Proposed four biobricks
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''K343000'' – FlhDCmut coding sequence <br><br>
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''K343000'' – ''FlhDCmut'' coding sequence <br><br>
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''K343001'' - Sandboxed coding sequence from ninaB gene. <br><br>
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''K343001'' - Sandboxed coding sequence from ''ninaB'' gene. <br><br>
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''K343002'' - Sandboxed coding sequence from ninaB gene on one of the weaker Anderson promoters, with rbs and dual terminator. <br><br>
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''K343002'' - Sandboxed coding sequence from ''ninaB'' gene on one of the weaker Anderson promoters, with rbs and dual terminator. <br><br>
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''K343003'' - Sandboxed coding sequence for the SopII-HtrII-Tsr fusion protein. <br><br>
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''K343003'' - Sandboxed coding sequence for the ''SopII-HtrII-Tsr'' fusion protein. <br><br>
''References:'' <br>
''References:'' <br>
5. ''Filling the gap in vitamin A research. Molecular identification of an enzyme cleaving beta-carotene to retinal.'',von Lintig J, Vogt K., J Biol Chem. 2000 Apr 21;275(16):11915-20. <br><br>
5. ''Filling the gap in vitamin A research. Molecular identification of an enzyme cleaving beta-carotene to retinal.'',von Lintig J, Vogt K., J Biol Chem. 2000 Apr 21;275(16):11915-20. <br><br>
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6. ''Analysis of the blind Drosophila mutant ninaB identifies the gene encoding the key enzyme for vitamin A formation in vivo'', Johannes von Lintig,* Armin Dreher, Cornelia Kiefer, Mathias F. Wernet, and Klaus Vogt, Proc Natl Acad Sci U S A. 2001 January 30; 98(3): 1130–1135. <br><br>
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6. ''Analysis of the blind ''Drosophila mutant'' ''ninaB'' identifies the gene encoding the key enzyme for vitamin A formation in vivo'', Johannes von Lintig,* Armin Dreher, Cornelia Kiefer, Mathias F. Wernet, and Klaus Vogt, Proc Natl Acad Sci U S A. 2001 January 30; 98(3): 1130–1135. <br><br>
== Modelling ==  
== Modelling ==  

Revision as of 15:58, 27 October 2010