Week 29

Flagella

• Extracted FlhDC master operon from E. coli strain MG1655
  
• Showed pressence of pst1 site in FlhC
  
• Introduced silent mutation into FlhC
  

Retinal

• Transformed the [http://partsregistry.org/Part:BBa_K274210 Cambridge part] in to Top10 cells
  
• Transformed an [http://partsregistry.org/Part:BBa_I0500 inducible promoter] in to Top10 cells
  
• Transformed [http://partsregistry.org/Part:BBa_E0040 GFP] in to MG1655 cells
  

http://igem.sdu.dk/wp-content/uploads/sponsor-sdu.png http://igem.sdu.dk/wp-content/uploads/sponsor-fermentas.png http://igem.sdu.dk/wp-content/uploads/sponsor-dnatech.png

Week 29

Flagella

• Made FlhDCmut
  

Retinal

• Ligated BBa_J04450 into pSB3C5 and pSB3T5, and transformed the plasmid into Top10 cells
  
• Ligated BBa_R0011 into pSB1A2 and transformed the plasmid into top10 cells
  

The iGEM team had a fun afternoon with many other students when it was "kindergarden day" at the students assiciation on the BMB institute. There were cake, popcorn and juice. You could play different games, like Mario Cart on Nintendo 64 and Kalaha, some painted their own coffee mug and Louise made a plastic pearl iGEM DENMARK logo. She didn't realize she had written 21010 instead of 2010 untill she had melted the pearls together though...rookie mistake