Team:SDU-Denmark/labnotes9

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(Difference between revisions)
(Miniprep of PS+B0015 sample Q and pSB3T5 containing J13002)
(Miniprep of PS+B0015 sample Q and pSB3T5 containing J13002)
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Sample Q did not fulfill DNA technologies sample specifications for sequencing we waited with sending it in, since we were expecting the results from sample C which was sent in earlier. Since those returned positive, sample Q was never sent in, since we just proceeded with sample C.
Sample Q did not fulfill DNA technologies sample specifications for sequencing we waited with sending it in, since we were expecting the results from sample C which was sent in earlier. Since those returned positive, sample Q was never sent in, since we just proceeded with sample C.
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=== Control restriction digest of PS+B0015 in pSB1AK3 sample Q, miniprep ===
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'''Date:''' 09/14<br>
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'''Done by:''' LC<br>
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'''Methods:''' Restriction digest<br>
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'''Protocols:''' RD1.1[https://2010.igem.org/Team:SDU-Denmark/protocols#RD1.1]<br>
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'''Notes:''' <br>
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The afore mentioned miniprep of PS+B0015 in pSB1AK3 (Q) was cut with EcoRI and PstI to check if biobrick pre- and suffix are present and the insert is the right size. <br>
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Mix for 1 RD reaction:<br>
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12 ul H2O<br>
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1 ul PstI<br>
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1 ul EcoRI <bR>
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2 ul Fast digest green buffer<br>
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5 ul PCR product<br>
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Uncut PCR product was loaded on the gel as a control.<br>
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Results: The RD resulted in three bands, uncut plasmid, plasmid and insert. All of those were the expected lengths, so the sample should be OK. <br>
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Revision as of 07:08, 28 September 2010