Team:SDU-Denmark/labnotes9

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(Flagella Group)
(Colony PCR of FlhDCmut in pSB1AK3 and pSB1C3)
 
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'''Results:''' <Br>
'''Results:''' <Br>
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=== Colony PCR of FlhDCmut in pSB1AK3 and pSB1C3 ===
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<br>
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'''Date:''' 9/15 2010<Br>
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'''Done By:''' Louise<Br>
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'''Protocol:''' [https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.3 CP1.3]<Br>
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'''Notes:''' We made 8 C-PCRs with colonies transformed with pSB1AK3 and 7 C-PCRs with colonies transformed with pSB1C3 (we made 8 but due to a mishab sample 5 was not run. Taq polymerase, VF2- and VR primers were used. Only Elongation time was altered from 2 min in the protocol to 1.5 min<Br>
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'''Results:''' <Br>
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The picture first picture is of FlhDCmut in pSB1AK3. This shows one band at about 400bp, the double terminator. This indicates that non of the colonies had the right plasmid.<br>
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The second picture is of FlhDCmut in pSB1C3.This shows the 4 of the 7 colonies had plasmids containing the right incert (sample 1,3, 7 and 8). These samples show a band at approximately 1200bp (FlhDCmut in pSB1C3 is 1248bp). the three other samples show a band heavier than 2000bp <br>
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[[Image:Team SDU-Denmark FlhDCmut indsat 1. gang i 1AK3 og 1C3.jpg|300px]]
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<br>
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----
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=== Colony PCR of FlhDCmut in pSC1AK3 ===
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<br>
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'''Date:''' 9/15 2010<Br>
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'''Done By:''' Louise<Br>
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'''Protocol:''' [https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.3 CP1.3]<Br>
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'''Notes:''' We made 8 C-PCRs with colonies transformed with pSB1AK3 and 7 C-PCRs with colonies transformed with pSB1C3 (we made 8 but due to a mishab sample 5 was not run. Taq polymerase, VF2- and VR primers were used. Only Elongation time was altered from 2 min in the protocol to 1.5 min<Br>
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'''Results:''' <Br>
== Photosensor group ==
== Photosensor group ==

Latest revision as of 10:23, 1 October 2010