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Team:SDU-Denmark/labnotes9
From 2010.igem.org
(Difference between revisions)
(→Restriction Digest of Gel Extracted FlhDCmut and pSB1C3 and pSB1AK3) |
(→Restriction Digest of Gel Extracted FlhDCmut and pSB1C3 and pSB1AK3) |
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== Flagella Group == | == Flagella Group == | ||
| - | === Restriction Digest of Gel Extracted FlhDCmut | + | === Restriction Digest of Gel Extracted FlhDCmut, pSB1C3 and pSB1AK3 === |
'''Date:''' 9/12 2010<Br> | '''Date:''' 9/12 2010<Br> | ||
'''Done By:''' Sheila<Br> | '''Done By:''' Sheila<Br> | ||
'''Protocol:''' [https://2010.igem.org/Team:SDU-Denmark/protocols#RD1.1 RD1.1]<Br> | '''Protocol:''' [https://2010.igem.org/Team:SDU-Denmark/protocols#RD1.1 RD1.1]<Br> | ||
| - | '''Notes:''' < | + | '''Notes:''' The restriction digests are made in preperation for two different ligations, the [https://2010.igem.org/Team:SDU-Denmark/labnotes9#Ligation_of_FlhDCmut_and_pSB1C3 first] is the ligation of FlhDCmut into the submission backbone; pSB1C3. The [https://2010.igem.org/Team:SDU-Denmark/labnotes9#Ligation_of_FlhDCmut_and_pSB1AK3 second] is the the ligation of FlhDCmut into pSB1AK3. Different restriction enzymes were used in, as shown in the following tables<br><br> |
''Restriction Digest mixtures''<Br> | ''Restriction Digest mixtures''<Br> | ||
<html> | <html> | ||
| Line 156: | Line 156: | ||
</body> | </body> | ||
</html> | </html> | ||
| + | <br> | ||
| + | Following digestion, the samples were loaded and run on a 1.5% agarose gel, before being extracted prior to ligation. | ||
'''Results:''' <Br> | '''Results:''' <Br> | ||
<Br><Br> | <Br><Br> | ||
Revision as of 18:08, 12 September 2010
