Team:SDU-Denmark/labnotes7

From 2010.igem.org

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(Colony PCR on B0017)
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Results: [[Image:Team-SDU-Denmark-cPCRB0017.jpg|300px]] No useable results, only unclear bands around 120 BP, which seem to be the result of mispriming with VR on B0010.
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Results: <br>
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[[Image:Team-SDU-Denmark-cPCRB0017.jpg|300px]] <br>
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No useable results, only unclear bands around 120 BP, which seem to be the result of mispriming with VR on B0010.
== Retinal ==
== Retinal ==
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Done by: Marie & Tommy<br>
Done by: Marie & Tommy<br>
Methods: PCR<br>
Methods: PCR<br>
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protocos:CP.1.1[https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1]
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protocos:[https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP1.1]
Notes:<br>
Notes:<br>
NinB2fw and NinaB2rv was used.<br>
NinB2fw and NinaB2rv was used.<br>
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Start date: 24/8<br>  
Start date: 24/8<br>  
Methods: Ligation, Competent cells, Transformation<br>
Methods: Ligation, Competent cells, Transformation<br>
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Protocols: LG1.1[https://2010.igem.org/Team:SDU-Denmark/protocols#LG1.1], CC1.1[https://2010.igem.org/Team:SDU-Denmark/protocols#CC1.1], TR1.1[https://2010.igem.org/Team:SDU-Denmark/protocols#TR1.1]
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Protocols: [https://2010.igem.org/Team:SDU-Denmark/protocols#LG1.1 LG1.1], [https://2010.igem.org/Team:SDU-Denmark/protocols#CC1.1 CC1.1], [https://2010.igem.org/Team:SDU-Denmark/protocols#TR1.1 TR1.1]
==== DNA purification from PCR ====
==== DNA purification from PCR ====
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Done by: Marie & Tommy<br>
Done by: Marie & Tommy<br>
Methods: Ligation<br>
Methods: Ligation<br>
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protocos:L1.3[https://2010.igem.org/Team:SDU-Denmark/protocols#LG1.3]
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protocos:[https://2010.igem.org/Team:SDU-Denmark/protocols#LG1.3 L1.3]
Notes:<br>
Notes:<br>
3 ligatons mixtures was made:<br>
3 ligatons mixtures was made:<br>
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Done by: Marie & Tommy<br>
Done by: Marie & Tommy<br>
Methods: Colony PCR<br>
Methods: Colony PCR<br>
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protocos:CP1.1[https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1]
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protocos:[https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP1.1]
Notes:<br>
Notes:<br>
15 colonies were picked form different plates, with differnt plasmid to insert ratio: colonie's 1,2,3,13 were picked from plates with 1:1 plasmid to insert ratio, colonie's 4,5,6 were picked from plates with 1:3 plasmid to insert ratio and colonie's 7,8,9,10,11,12,14,15 were picked from plates with 1:6 plasmid to insert ratio.<br>
15 colonies were picked form different plates, with differnt plasmid to insert ratio: colonie's 1,2,3,13 were picked from plates with 1:1 plasmid to insert ratio, colonie's 4,5,6 were picked from plates with 1:3 plasmid to insert ratio and colonie's 7,8,9,10,11,12,14,15 were picked from plates with 1:6 plasmid to insert ratio.<br>
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Done by: Marie & Tommy<br>
Done by: Marie & Tommy<br>
Methods: Restriction Digest<br>
Methods: Restriction Digest<br>
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protocos:RD1.1[https://2010.igem.org/Team:SDU-Denmark/protocols#RD1.1]
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protocos:[https://2010.igem.org/Team:SDU-Denmark/protocols#RD1.1 RD1.1]
Notes:<br>
Notes:<br>
Restriction digest was performed on tubes 5,6,10 and 11 to test for insertion of ninaB (Correct orientation) XbaI and SPEI was used and a gel was run:<br>
Restriction digest was performed on tubes 5,6,10 and 11 to test for insertion of ninaB (Correct orientation) XbaI and SPEI was used and a gel was run:<br>
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Done by: Marie & Tommy<br>
Done by: Marie & Tommy<br>
Methods: Colony PCR<br>
Methods: Colony PCR<br>
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protocos:CP1.1[https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1]
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protocos:[https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP1.1]
Notes:<br>
Notes:<br>
8 new colonies (16-23) were chosen in addition to colonies 5,6,10 and 11 form 25I8-10 colonie PCR.<br>
8 new colonies (16-23) were chosen in addition to colonies 5,6,10 and 11 form 25I8-10 colonie PCR.<br>
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Latest revision as of 22:31, 23 October 2010