Team:SDU-Denmark/labnotes4

From 2010.igem.org

(Difference between revisions)
(Group: Flagella)
(Insertion of B0015 in pSB3C5)
Line 277: Line 277:
''Analysis:''
''Analysis:''
PCR product is OK and B0015 DNA is purified from the PCR product according to protocol. DNA is eluted in 20uL H2O. Purified samples are pooled and used for digestion.<br><br>
PCR product is OK and B0015 DNA is purified from the PCR product according to protocol. DNA is eluted in 20uL H2O. Purified samples are pooled and used for digestion.<br><br>
 +
 +
==== Digestion of B0015 and pSB3C5 ====
 +
''date:''4/8<br>
 +
''Protocols:''[https://2010.igem.org/Team:SDU-Denmark/protocols#RD1.1 RD1.1][https://2010.igem.org/Team:SDU-Denmark/protocols#DE1.3 DE1.3]<br>
 +
''Notes:''<br>
 +
purified B0015 PCR product and mini prep of pSB3C5 (28 white) are digested with EcoRI and PstI.<br>
 +
Restriction mixture:<br>
 +
<table style="text-align: left; width: 100%;" border="1"
 +
cellpadding="2" cellspacing="2">
 +
    <tr>
 +
      <td>Restriction mixture B0015 </td>
 +
      <td></td>
 +
      <td>Restriction mixture Plasmid</td>
 +
    </tr>
 +
    <tr>
 +
      <td>38uL</td>
 +
      <td>H2O</td>
 +
      <td>24uL</td>
 +
    </tr>
 +
    <tr>
 +
      <td>8uL</td>
 +
      <td>FD green buffer</td>
 +
      <td>4uL</td>
 +
    </tr>
 +
    <tr>
 +
      <td>4uL</td>
 +
      <td>EcoRI</td>
 +
      <td>2uL</td>
 +
    </tr>
 +
    <tr>
 +
      <td>4uL</td>
 +
      <td>PstI</td>
 +
      <td>2uL</td>
 +
    </tr>
 +
    <tr>
 +
      <td>30uL</td>
 +
      <td>DNA</td>
 +
      <td>10uL</td>
 +
    </tr>
 +
</table><br>
 +
Total volume of digested samples were loaded onto agarose gels and extracted from gel according to protocol. DNA was eluted in 20uL H2O.<br><br>
 +
''Results:''<br>
 +
<br><br>
 +
''Analysis:''<br>
 +
Digested DNA was used for ligation.<br><br>
== Group: Photosensor ==
== Group: Photosensor ==

Revision as of 09:43, 16 August 2010