Team:SDU-Denmark/labnotes4

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(vol. 3)
(Colony PCR of FlhDCmut and DT (B0015))
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'''''FlhDCmut in pSB1c3:'''''
'''''FlhDCmut in pSB1c3:'''''
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=== Colony PCR of pSB3T3 w RFP(J04450) ===
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''Done by:'' Pernille <br>
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''Date:'' August 10th <br>
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''Protocol:'' [https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP1.1] <br>
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''Notes:'' The experiment was done with Taq will the purpose only was to check if  the RFP was amplificated when using the primers VR og VF2. For the PCR i used the mini prep product from freeze tube 29. The biobrick in in the pSB3T3 plamid are 1319bp long. <br>
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''Results:'' [[Image:Team-SDU-Denmark-psb3T3wRFP.jpg | 400px]]<br>
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Unfortunately there was no band around 1400bp and therefore the amplification was not succeed. the upper band is probably the plamid.
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--[[User:Pernm07|Pernm07]] 08:58, 11 August 2010 (UTC)
== Group: Photosensor ==
== Group: Photosensor ==

Revision as of 08:58, 11 August 2010