Team:SDU-Denmark/labnotes3

From 2010.igem.org

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(compent cell and transformation of photosensor and promotor+RNA in pSB3T5)
(PCR of FlhDC freeye tube 23)
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=== PCR of FlhDC freeye tube 23 ===
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=== PCR of FlhDC freeze tube 23 ===
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''Done bz:'' Louise <br>
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''Done by:'' Louise <br>
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''Date:'' julz 29th <br>
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''Date:'' july 29th <br>
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''Protcol:'' [https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP1.1]
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''Protcol:'' [https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP1.1] <br>
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''Notes:'' Since the PCR of freeze tube 31 shoved pour results on the gel, we are now trying another sample (freeze tube 23) This is made from sample 14 and 16 (mutation PCR samples). The gel picture from these samples showed the strongest band in the samples run with annealing temperature of 64.5 celcius.<br><br>
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''Results:'' The PCR result was pooled in freeze tube 49.<br>
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[[Image:Team-SDU Denmark Double terminator and flhdc freeze tube 49.jpg]]
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<br> The picture shows a clear band at 200bp and smear. The FlhDC gene is 986bp.
== Follow-up colony PCR ===
== Follow-up colony PCR ===

Revision as of 13:16, 3 August 2010