Team:SDU-Denmark/labnotes3

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(Restriction digest of PCR product from 26/7)
(colony PCR using taq on ligated BBa_J13002 in pSB1C3)
 
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Results: A band showed at around 300bp in well 4.1. The expected length was 312bp. wells3.3 and 3.4 show a band outside the blue ladder, that could be rfp (since this was the original insert.) tube 3.2 was ruined.<br>
Results: A band showed at around 300bp in well 4.1. The expected length was 312bp. wells3.3 and 3.4 show a band outside the blue ladder, that could be rfp (since this was the original insert.) tube 3.2 was ruined.<br>
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[[Image:Team-sdu-denmark-Promotor_Rbs_i_psb3t5_colonipcr.jpg | 400px]]
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[[Image:Team-sdu-denmark-Promotor_Rbs_i_psb3t5_colonipcr.jpg | 300px]]
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Analysis: Well 3.2 shows promising results. ON and miniprep will be made, and it will be sent for sequencing.<br><br>
Analysis: Well 3.2 shows promising results. ON and miniprep will be made, and it will be sent for sequencing.<br><br>
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''Notes:'' purified flhD/C and miniprep of pSB1C3 and pSB3K3 were digested using the EcoRI and PstI restriction enzymes. <br><br>
''Notes:'' purified flhD/C and miniprep of pSB1C3 and pSB3K3 were digested using the EcoRI and PstI restriction enzymes. <br><br>
Restriction mixture (FlhD/C):<br>
Restriction mixture (FlhD/C):<br>
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Restriction mixture (plasmid):<br>
Restriction mixture (plasmid):<br>
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DNA was extracted from gel according to protocol<br><br>
DNA was extracted from gel according to protocol<br><br>
''Results:''<br><br>
''Results:''<br><br>
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==== Ligation ====
==== Ligation ====
''Protocol:'' [https://2010.igem.org/Team:SDU-Denmark/protocols#LG1.2 LG1.2]<br><br>
''Protocol:'' [https://2010.igem.org/Team:SDU-Denmark/protocols#LG1.2 LG1.2]<br><br>
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=== Follow-up colony PCR ===
=== Follow-up colony PCR ===
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Date: 26/7<br>
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''Date:'' 26/7<br>
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Methods: Colony PCR<br>
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''Methods:'' Colony PCR<br>
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Protocol: CP1.3<br>
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''Protocol:'' CP1.3<br>
Experiment done by: Maria, LC  
Experiment done by: Maria, LC  
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Notes: We made a sample for every plate from the last colony PCR. Out of the 29 samples we could only run 25 at once in the PCR machine. Plate 23 was missing, so there is no sample for it.<br><br>
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''Notes:'' We made a sample for every plate from the last colony PCR. Out of the 29 samples we could only run 25 at once in the PCR machine. Plate 23 was missing, so there is no sample for it.<br><br>
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Results: [[Image:Team-SDU-Denmark-colpcr267.jpg|600px]]<br>
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''Results:'' <br>
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[[Image:Team-SDU-Denmark-colpcr267.jpg|300px]]<br>
Lengths of PCR products: <br>
Lengths of PCR products: <br>
1200: 4, 5, 6, 9, 10, 14, 21, 26<br>
1200: 4, 5, 6, 9, 10, 14, 21, 26<br>
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Colonies 1, 15, 16 and 22 gave no result.
Colonies 1, 15, 16 and 22 gave no result.
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Analysis: Since we had so many different lengths, we will cut one from each length, specifically colony: 2, 8, 11, 13, 20, 24, 26.
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''Analysis:'' Since we had so many different lengths, we will cut one from each length, specifically colony: 2, 8, 11, 13, 20, 24, 26.
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Latest revision as of 21:27, 23 October 2010