http://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/labnotes2&feed=atom&action=historyTeam:SDU-Denmark/labnotes2 - Revision history2024-03-28T17:22:41ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/labnotes2&diff=136491&oldid=prevJanni Vester Bjerrum: /* Group: Retinal */2010-10-24T19:23:45Z<p><span class="autocomment">Group: Retinal</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Group: Retinal ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Group: Retinal ==</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=== Transformation of [http://partsregistry.org/Part:BBa_K081005 <del class="diffchange diffchange-inline">BBa_K081005</del>] in pSB1A2 (constitutive promoter and RBS combined),[http://partsregistry.org/Part:BBa_R0011 <del class="diffchange diffchange-inline">BBa_R0011</del>] in pSB1A2, pSB3C5 w. [http://partsregistry.org/Part:BBa_J04450 <del class="diffchange diffchange-inline">BBa_J04450</del>] and pSB3T5 w. <del class="diffchange diffchange-inline">BBa_J04450 </del>in Top 10 E.Coli ===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=== Transformation of [http://partsregistry.org/Part:BBa_K081005 <ins class="diffchange diffchange-inline">K081005</ins>] in pSB1A2 (constitutive promoter and RBS combined),[http://partsregistry.org/Part:BBa_R0011 <ins class="diffchange diffchange-inline">R0011</ins>] in pSB1A2, pSB3C5 w. [http://partsregistry.org/Part:BBa_J04450 <ins class="diffchange diffchange-inline">J04450</ins>] and pSB3T5 w. <ins class="diffchange diffchange-inline">J04450 </ins>in Top 10 E.Coli ===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Start date: 19/07 End date: 20/07<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Start date: 19/07 End date: 20/07<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' ON culture, making competent cells, transformation <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' ON culture, making competent cells, transformation <br><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>--[[User:Tipi|Tipi]] 08:17, 21 July 2010 (UTC)<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>--[[User:Tipi|Tipi]] 08:17, 21 July 2010 (UTC)<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=== Coloni PCR of [http://partsregistry.org/Part:BBa_R0011 <del class="diffchange diffchange-inline">BBa_R0011</del>] in pSB1A2 and [http://partsregistry.org/Part:BBa_K081005 <del class="diffchange diffchange-inline">BBa_K081005</del>] in pSB1A2 ===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=== Coloni PCR of [http://partsregistry.org/Part:BBa_R0011 <ins class="diffchange diffchange-inline">R0011</ins>] in pSB1A2 and [http://partsregistry.org/Part:BBa_K081005 <ins class="diffchange diffchange-inline">K081005</ins>] in pSB1A2 ===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Start date: 20/07 End date: 20/07<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Start date: 20/07 End date: 20/07<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' Coloni PCR and gel electrophoresis <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' Coloni PCR and gel electrophoresis <br><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Experiment done by:'' Maria and LC<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Experiment done by:'' Maria and LC<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Notes:''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Notes:''<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>6 colonies transformed with pSB1A2 w. <del class="diffchange diffchange-inline">BBa_R0011 </del>and 4 colonies transformed with pSB1A2 w. <del class="diffchange diffchange-inline">BBa_K081005 </del>(see [https://2010.igem.org/Team:SDU-Denmark/labnotes2#Transformation_of_K081005_in_pSB1A2_.28constitutive_promoter_and_RBS_combined.29.2CR0011_in_pSB1A2.2C_pSB3C5_w._J04450_and_pSB3T5_w._J04450_in_Top_10_E.Coli Transformation]) was used for coloni PCR. <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>6 colonies transformed with pSB1A2 w. <ins class="diffchange diffchange-inline">R0011 </ins>and 4 colonies transformed with pSB1A2 w. <ins class="diffchange diffchange-inline">K081005 </ins>(see [https://2010.igem.org/Team:SDU-Denmark/labnotes2#Transformation_of_K081005_in_pSB1A2_.28constitutive_promoter_and_RBS_combined.29.2CR0011_in_pSB1A2.2C_pSB3C5_w._J04450_and_pSB3T5_w._J04450_in_Top_10_E.Coli Transformation]) was used for coloni PCR. <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Colonies with K081005 was denoted A1, A2 A3 and A4 respectively.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Colonies with K081005 was denoted A1, A2 A3 and A4 respectively.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Colonies with R0011 was denotes B1, B2, B3, B4, B5 and B6 respectively<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Colonies with R0011 was denotes B1, B2, B3, B4, B5 and B6 respectively<br></div></td></tr>
</table>Janni Vester Bjerrumhttp://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/labnotes2&diff=136460&oldid=prevJanni Vester Bjerrum: /* Group: Photosensor */2010-10-24T19:20:30Z<p><span class="autocomment">Group: Photosensor</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Group: Photosensor ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Group: Photosensor ==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=== Miniprep of [http://partsregistry.org/Part:BBa_K081005 <del class="diffchange diffchange-inline">BBa_K081005</del>] in pSB1A2 and [http://partsregistry.org/Part:BBa_R0011 <del class="diffchange diffchange-inline">BBa_R0011</del>] in pSB1A2 from transformation 20/7 ===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=== Miniprep of [http://partsregistry.org/Part:BBa_K081005 <ins class="diffchange diffchange-inline">K081005</ins>] in pSB1A2 and [http://partsregistry.org/Part:BBa_R0011 <ins class="diffchange diffchange-inline">R0011</ins>] in pSB1A2 from transformation 20/7 ===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Start date: July 22nd<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Start date: July 22nd<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' Miniprep, gel electrophoresis and nano drop <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' Miniprep, gel electrophoresis and nano drop <br><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Analysis:''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Analysis:''<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Sample 1-4 of <del class="diffchange diffchange-inline">BBa_K081005 </del>were pooled and frozen and sample 1-4 of <del class="diffchange diffchange-inline">BBa_R0011 </del>were too.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Sample 1-4 of <ins class="diffchange diffchange-inline">K081005 </ins>were pooled and frozen and sample 1-4 of <ins class="diffchange diffchange-inline">R0011 </ins>were too.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
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</table>Janni Vester Bjerrumhttp://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/labnotes2&diff=120743&oldid=prevJanni Vester Bjerrum: /* Group: Photosensor */2010-10-22T17:01:35Z<p><span class="autocomment">Group: Photosensor</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Group: Photosensor ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Group: Photosensor ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Miniprep of [http://partsregistry.org/Part:BBa_K081005 BBa_K081005] in pSB1A2 and [http://partsregistry.org/Part:BBa_R0011 BBa_R0011] in pSB1A2 from transformation 20/7 ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Miniprep of [http://partsregistry.org/Part:BBa_K081005 BBa_K081005] in pSB1A2 and [http://partsregistry.org/Part:BBa_R0011 BBa_R0011] in pSB1A2 from transformation 20/7 ===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Start date: <del class="diffchange diffchange-inline">22/07 End date: 22/07</del><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Start date: <ins class="diffchange diffchange-inline">July 22nd</ins><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' Miniprep, gel electrophoresis and nano drop <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' Miniprep, gel electrophoresis and nano drop <br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Protocol:''[https://2010.igem.org/Team:SDU-Denmark/protocols#MP1.2 MP1.2] <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Protocol:''[https://2010.igem.org/Team:SDU-Denmark/protocols#MP1.2 MP1.2] <br><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Gel extraction test (GFX vs. Fermentas) ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Gel extraction test (GFX vs. Fermentas) ===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Start date: <del class="diffchange diffchange-inline">25/07 End date: 25/07</del><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Start date: <ins class="diffchange diffchange-inline">July 25th </ins><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' Gel electrophoresis, gel extraction and nano drop <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' Gel electrophoresis, gel extraction and nano drop <br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Protocol:''[https://2010.igem.org/Team:SDU-Denmark/protocols#DE1.2 DE1.2][https://2010.igem.org/Team:SDU-Denmark/protocols#DE1.3 DE1.3] <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Protocol:''[https://2010.igem.org/Team:SDU-Denmark/protocols#DE1.2 DE1.2][https://2010.igem.org/Team:SDU-Denmark/protocols#DE1.3 DE1.3] <br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Experiment done by:'' Maria<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Experiment done by:'' Maria<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Notes:'' <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Notes:'' <br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>FlhD/C <del class="diffchange diffchange-inline">tq </del>PCR product (5 green) is used in the test.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>FlhD/C <ins class="diffchange diffchange-inline">taq </ins>PCR product (5 green) is used in the test.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR product is diluted in H2O to reach a sample concentration below 100ng/uL.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PCR product is diluted in H2O to reach a sample concentration below 100ng/uL.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Nanodrop:<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Nanodrop:<br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></tr></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></table><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></table><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>A total of <del class="diffchange diffchange-inline">70uL </del>is loaded onto a 2% agarose gel.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>A total of <ins class="diffchange diffchange-inline">70 microlitres </ins>is loaded onto a 2% agarose gel.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the GFX gel extraction 350mg of gel was used.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the GFX gel extraction 350mg of gel was used.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the Fermentas extraction 460mg of gel was used.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the Fermentas extraction 460mg of gel was used.<br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Analysis:''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Analysis:''<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">There </del>is extracted <del class="diffchange diffchange-inline">more DNA </del>with the fermentas kit. This may be due to the short centrifugation time in the GFX protocol. Furthermore the GFX kit that was used was old and the buffers may have been contaminated.<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">More DNA </ins>is extracted with the fermentas kit. This may be due to the short centrifugation time in the GFX protocol. Furthermore the GFX kit that was used was old and the buffers may have been contaminated.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Group: Retinal ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Group: Retinal ==</div></td></tr>
</table>Janni Vester Bjerrumhttp://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/labnotes2&diff=120573&oldid=prevJanni Vester Bjerrum: /* Amplification of FlhDCmut */2010-10-22T16:08:58Z<p><span class="autocomment">Amplification of FlhDCmut</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 16:08, 22 October 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Date:'' July 19th <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Date:'' July 19th <br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Protocol:'' [https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP1.1]<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Protocol:'' [https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP1.1]<br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>''Method:'' The experiment was done in two rounds. First, the annealing product, made by Pernille on <del class="diffchange diffchange-inline">july </del>19th was amplified. Then we amplified Pernilles amplification simultaneously as we amplified [https://2010.igem.org/Team:SDU-Denmark/labnotes2#Annealing_of_the_two_mutated_strands_of_FlhDC_.28FlhDCmut.29 Sheilas previous annealing].<br> Primers used: FlhDC fw and FlhDC rev <br> Polymerase used: Pfu<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>''Method:'' The experiment was done in two rounds. First, the annealing product, made by Pernille on <ins class="diffchange diffchange-inline">July </ins>19th was amplified. Then we amplified Pernilles amplification simultaneously as we amplified [https://2010.igem.org/Team:SDU-Denmark/labnotes2#Annealing_of_the_two_mutated_strands_of_FlhDC_.28FlhDCmut.29 Sheilas previous annealing].<br> Primers used: FlhDC fw and FlhDC rev <br> Polymerase used: Pfu<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Notes:'' <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Notes:'' <br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Results:''<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Results:''<br><br></div></td></tr>
</table>Janni Vester Bjerrumhttp://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/labnotes2&diff=120570&oldid=prevJanni Vester Bjerrum: /* Annealing of the two mutated strands of FlhDC (FlhDCmut) */2010-10-22T16:07:32Z<p><span class="autocomment">Annealing of the two mutated strands of FlhDC (FlhDCmut)</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>''Results:'' A gel, containing the PCR product was run, using [http://www.fermentas.de/product_info.php?info=p1110 GeneRuler(TM) DNA Ladder Mix, ready-to-use (#SM0333)] loaded in lane one <del class="diffchange diffchange-inline">(from the left: Marker</del>, sample at 56,1 degrees celcius<del class="diffchange diffchange-inline">, </del>sample at 64,5 degrees celcius<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>''Results:'' A gel, containing the PCR product was run, using [http://www.fermentas.de/product_info.php?info=p1110 GeneRuler(TM) DNA Ladder Mix, ready-to-use (#SM0333)] loaded in lane one, <ins class="diffchange diffchange-inline">lane 2 contains </ins>sample <ins class="diffchange diffchange-inline">run </ins>at 56,1 degrees celcius <ins class="diffchange diffchange-inline">and in lane 3 </ins>sample <ins class="diffchange diffchange-inline">run </ins>at 64,5 degrees celcius <ins class="diffchange diffchange-inline">is found.</ins><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Annealing of the two mutated FlhDC strands (sheila).jpg|300px]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Annealing of the two mutated FlhDC strands (sheila).jpg|300px]]<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We can se very faint bands in lane 2 and 3, at around 1000bp. The band in lane 3 is a little more visible than lane 2. Because we haven't used primers <del class="diffchange diffchange-inline">ind </del>this PCR, <del class="diffchange diffchange-inline">it is possible that </del>the <del class="diffchange diffchange-inline">two mutated strands has a lower possibility of annealing </del>to <del class="diffchange diffchange-inline">each other. It s however possible </del>to <del class="diffchange diffchange-inline">amplify the few complete FlhDCmut strands in a new PCR, which is what we will do next</del>.<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We can se very faint bands in lane 2 and 3, at around 1000bp<ins class="diffchange diffchange-inline">, which is the right size</ins>. The band in lane 3 is a little more visible than lane 2. Because we haven't used primers <ins class="diffchange diffchange-inline">in </ins>this PCR, the <ins class="diffchange diffchange-inline">pieces will only have been extended once and we need </ins>to <ins class="diffchange diffchange-inline">ad flhDC fw and rv in order </ins>to <ins class="diffchange diffchange-inline">obtain more product</ins>. <br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>--[[User:Sheila|Sheila]] 09:10, 22 July 2010 (UTC)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>--[[User:Sheila|Sheila]] 09:10, 22 July 2010 (UTC)</div></td></tr>
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</table>Janni Vester Bjerrumhttp://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/labnotes2&diff=120550&oldid=prevJanni Vester Bjerrum: /* Annealing of the two mutated strands of FlhDC (FlhDCmut) */2010-10-22T16:04:10Z<p><span class="autocomment">Annealing of the two mutated strands of FlhDC (FlhDCmut)</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>''Results:'' A gel, containing the PCR product was run, using [http://www.fermentas.de/product_info.php?info=p1110 GeneRuler(TM) DNA Ladder Mix, ready-to-use (#SM0333)] loaded in lane one (from the left:<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>''Results:'' A gel, containing the PCR product was run, using [http://www.fermentas.de/product_info.php?info=p1110 GeneRuler(TM) DNA Ladder Mix, ready-to-use (#SM0333)] loaded in lane one (from the left: <ins class="diffchange diffchange-inline">Marker, sample at 56,1 degrees celcius, sample at 64,5 degrees celcius</ins><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Annealing of the two mutated FlhDC strands (sheila).jpg|300px]]<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Annealing of the two mutated FlhDC strands (sheila).jpg|300px]]<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We can se very faint bands in lane 2 and 3, at around 1000bp. The band in lane 3 is a little more visible than lane 2. Because we haven't used primers ind this PCR, it is possible that the two mutated strands has a lower possibility of annealing to each other. It s however possible to amplify the few complete FlhDCmut strands in a new PCR, which is what we will do next.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We can se very faint bands in lane 2 and 3, at around 1000bp. The band in lane 3 is a little more visible than lane 2. Because we haven't used primers ind this PCR, it is possible that the two mutated strands has a lower possibility of annealing to each other. It s however possible to amplify the few complete FlhDCmut strands in a new PCR, which is what we will do next.<br><br></div></td></tr>
</table>Janni Vester Bjerrumhttp://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/labnotes2&diff=117862&oldid=prevLouch07: /* Coloni PCR of R0011 in pSB1A2 and K081005 in pSB1A2 */2010-10-21T20:17:55Z<p><span class="autocomment">Coloni PCR of R0011 in pSB1A2 and K081005 in pSB1A2</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>--[[User:Tipi|Tipi]] 08:17, 21 July 2010 (UTC)<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>--[[User:Tipi|Tipi]] 08:17, 21 July 2010 (UTC)<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=== Coloni PCR of <del class="diffchange diffchange-inline">R0011 </del>in pSB1A2 and <del class="diffchange diffchange-inline">K081005 </del>in pSB1A2 ===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=== Coloni PCR of <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_R0011 BBa_R0011] </ins>in pSB1A2 and <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_K081005 BBa_K081005] </ins>in pSB1A2 ===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Start date: 20/07 End date: 20/07<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Start date: 20/07 End date: 20/07<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' Coloni PCR and gel electrophoresis <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' Coloni PCR and gel electrophoresis <br><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Experiment done by:'' Maria and LC<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Experiment done by:'' Maria and LC<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Notes:''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Notes:''<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>6 colonies transformed with pSB1A2 w. <del class="diffchange diffchange-inline">R0011 </del>and 4 colonies transformed with pSB1A2 w. <del class="diffchange diffchange-inline">K081005 </del>(see [https://2010.igem.org/Team:SDU-Denmark/labnotes2#Transformation_of_K081005_in_pSB1A2_.28constitutive_promoter_and_RBS_combined.29.2CR0011_in_pSB1A2.2C_pSB3C5_w._J04450_and_pSB3T5_w._J04450_in_Top_10_E.Coli Transformation]) was used for coloni PCR. <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>6 colonies transformed with pSB1A2 w. <ins class="diffchange diffchange-inline">BBa_R0011 </ins>and 4 colonies transformed with pSB1A2 w. <ins class="diffchange diffchange-inline">BBa_K081005 </ins>(see [https://2010.igem.org/Team:SDU-Denmark/labnotes2#Transformation_of_K081005_in_pSB1A2_.28constitutive_promoter_and_RBS_combined.29.2CR0011_in_pSB1A2.2C_pSB3C5_w._J04450_and_pSB3T5_w._J04450_in_Top_10_E.Coli Transformation]) was used for coloni PCR. <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Colonies with K081005 was denoted A1, A2 A3 and A4 respectively.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Colonies with K081005 was denoted A1, A2 A3 and A4 respectively.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Colonies with R0011 was denotes B1, B2, B3, B4, B5 and B6 respectively<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Colonies with R0011 was denotes B1, B2, B3, B4, B5 and B6 respectively<br></div></td></tr>
</table>Louch07http://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/labnotes2&diff=117851&oldid=prevLouch07: /* Transformation of BBa_K081005 in pSB1A2 (constitutive promoter and RBS combined),BBa_R0011 in pSB1A2, pSB3C5 w. BBa_J04450 and pSB3T5 w. J04450 in Top 10 E.Coli */2010-10-21T20:13:39Z<p><span class="autocomment">Transformation of BBa_K081005 in pSB1A2 (constitutive promoter and RBS combined),BBa_R0011 in pSB1A2, pSB3C5 w. BBa_J04450 and pSB3T5 w. J04450 in Top 10 E.Coli</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Group: Retinal ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Group: Retinal ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=== Transformation of [http://partsregistry.org/Part:BBa_K081005 BBa_K081005] in pSB1A2 (constitutive promoter and RBS combined),[http://partsregistry.org/Part:BBa_R0011 BBa_R0011] in pSB1A2, pSB3C5 w. [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] and pSB3T5 w. <del class="diffchange diffchange-inline">J04450 </del>in Top 10 E.Coli ===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=== Transformation of [http://partsregistry.org/Part:BBa_K081005 BBa_K081005] in pSB1A2 (constitutive promoter and RBS combined),[http://partsregistry.org/Part:BBa_R0011 BBa_R0011] in pSB1A2, pSB3C5 w. [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] and pSB3T5 w. <ins class="diffchange diffchange-inline">BBa_J04450 </ins>in Top 10 E.Coli ===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Start date: 19/07 End date: 20/07<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Start date: 19/07 End date: 20/07<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' ON culture, making competent cells, transformation <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' ON culture, making competent cells, transformation <br><br></div></td></tr>
</table>Louch07http://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/labnotes2&diff=117850&oldid=prevLouch07: /* Transformation of K081005 in pSB1A2 (constitutive promoter and RBS combined),R0011 in pSB1A2, pSB3C5 w. J04450 and pSB3T5 w. J04450 in Top 10 E.Coli */2010-10-21T20:12:59Z<p><span class="autocomment">Transformation of K081005 in pSB1A2 (constitutive promoter and RBS combined),R0011 in pSB1A2, pSB3C5 w. J04450 and pSB3T5 w. J04450 in Top 10 E.Coli</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Group: Retinal ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Group: Retinal ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=== Transformation of <del class="diffchange diffchange-inline">K081005 </del>in pSB1A2 (constitutive promoter and RBS combined),<del class="diffchange diffchange-inline">R0011 </del>in pSB1A2, pSB3C5 w. <del class="diffchange diffchange-inline">J04450 </del>and pSB3T5 w. J04450 in Top 10 E.Coli ===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=== Transformation of <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_K081005 BBa_K081005] </ins>in pSB1A2 (constitutive promoter and RBS combined),<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_R0011 BBa_R0011] </ins>in pSB1A2, pSB3C5 w. <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_J04450 BBa_J04450] </ins>and pSB3T5 w. J04450 in Top 10 E.Coli ===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Start date: 19/07 End date: 20/07<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Start date: 19/07 End date: 20/07<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' ON culture, making competent cells, transformation <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' ON culture, making competent cells, transformation <br><br></div></td></tr>
</table>Louch07http://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/labnotes2&diff=117836&oldid=prevLouch07: /* Miniprep of BBa_K081005 in pSB1A2 and BBa_R0011 in pSB1A2 from transformation 20/7 */2010-10-21T20:08:13Z<p><span class="autocomment">Miniprep of BBa_K081005 in pSB1A2 and BBa_R0011 in pSB1A2 from transformation 20/7</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Group: Photosensor ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Group: Photosensor ==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=== Miniprep of BBa_K081005 in pSB1A2 and BBa_R0011 in pSB1A2 from transformation 20/7 ===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=== Miniprep of <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:</ins>BBa_K081005 <ins class="diffchange diffchange-inline">BBa_K081005] </ins>in pSB1A2 and <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_R0011 </ins>BBa_R0011<ins class="diffchange diffchange-inline">] </ins>in pSB1A2 from transformation 20/7 ===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Start date: 22/07 End date: 22/07<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Start date: 22/07 End date: 22/07<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' Miniprep, gel electrophoresis and nano drop <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Methods:'' Miniprep, gel electrophoresis and nano drop <br><br></div></td></tr>
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<td colspan="2" class="diff-lineno">Line 1,251:</td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Analysis:''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Analysis:''<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Sample 1-4 of BBa_K081005 were pooled and frozen <del class="diffchange diffchange-inline"> </del>and sample 1-4 of BBa_R0011 were too.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Sample 1-4 of BBa_K081005 were pooled and frozen and sample 1-4 of BBa_R0011 were too.<br></div></td></tr>
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</table>Louch07