Team:SDU-Denmark/labnotes2

From 2010.igem.org

(Difference between revisions)
(Annealing of the two mutated strands of FlhDC (FlhDCmut))
(Amplification of FlhDCmut)
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''Date:'' July 19th <br><br>
''Date:'' July 19th <br><br>
''Protocol:'' [https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP1.1]<br><br>
''Protocol:'' [https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP1.1]<br><br>
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''Method:'' The experiment was done in two rounds. First, the annealing product, made by Pernille on july 19th was amplified. Then we amplified Pernilles amplification simultaneously as we amplified [https://2010.igem.org/Team:SDU-Denmark/labnotes2#Annealing_of_the_two_mutated_strands_of_FlhDC_.28FlhDCmut.29 Sheilas previous annealing].<br> Primers used: FlhDC fw and FlhDC rev <br> Polymerase used: Pfu<br><br>
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''Method:'' The experiment was done in two rounds. First, the annealing product, made by Pernille on July 19th was amplified. Then we amplified Pernilles amplification simultaneously as we amplified [https://2010.igem.org/Team:SDU-Denmark/labnotes2#Annealing_of_the_two_mutated_strands_of_FlhDC_.28FlhDCmut.29 Sheilas previous annealing].<br> Primers used: FlhDC fw and FlhDC rev <br> Polymerase used: Pfu<br><br>
''Notes:'' <br><br>
''Notes:'' <br><br>
''Results:''<br><br>
''Results:''<br><br>

Revision as of 16:08, 22 October 2010