Team:SDU-Denmark/labnotes2

From 2010.igem.org

(Difference between revisions)
(Transformation of BBa_K081005 in pSB1A2 (constitutive promoter and RBS combined),BBa_R0011 in pSB1A2, pSB3C5 w. BBa_J04450 and pSB3T5 w. J04450 in Top 10 E.Coli)
(Coloni PCR of R0011 in pSB1A2 and K081005 in pSB1A2)
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--[[User:Tipi|Tipi]] 08:17, 21 July 2010 (UTC)<br><br>
--[[User:Tipi|Tipi]] 08:17, 21 July 2010 (UTC)<br><br>
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=== Coloni PCR of R0011 in pSB1A2 and K081005 in pSB1A2 ===
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=== Coloni PCR of [http://partsregistry.org/Part:BBa_R0011 BBa_R0011] in pSB1A2 and [http://partsregistry.org/Part:BBa_K081005 BBa_K081005] in pSB1A2 ===
Start date: 20/07    End date: 20/07<br>
Start date: 20/07    End date: 20/07<br>
''Methods:''  Coloni PCR and gel electrophoresis <br><br>
''Methods:''  Coloni PCR and gel electrophoresis <br><br>
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''Experiment done by:'' Maria and LC<br><br>
''Experiment done by:'' Maria and LC<br><br>
''Notes:''<br>
''Notes:''<br>
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6 colonies transformed with pSB1A2 w. R0011 and 4 colonies transformed with pSB1A2 w. K081005 (see [https://2010.igem.org/Team:SDU-Denmark/labnotes2#Transformation_of_K081005_in_pSB1A2_.28constitutive_promoter_and_RBS_combined.29.2CR0011_in_pSB1A2.2C_pSB3C5_w._J04450_and_pSB3T5_w._J04450_in_Top_10_E.Coli Transformation]) was used for coloni PCR. <br>
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6 colonies transformed with pSB1A2 w. BBa_R0011 and 4 colonies transformed with pSB1A2 w. BBa_K081005 (see [https://2010.igem.org/Team:SDU-Denmark/labnotes2#Transformation_of_K081005_in_pSB1A2_.28constitutive_promoter_and_RBS_combined.29.2CR0011_in_pSB1A2.2C_pSB3C5_w._J04450_and_pSB3T5_w._J04450_in_Top_10_E.Coli Transformation]) was used for coloni PCR. <br>
Colonies with K081005 was denoted A1, A2 A3 and A4 respectively.<br>
Colonies with K081005 was denoted A1, A2 A3 and A4 respectively.<br>
Colonies with R0011 was denotes B1, B2, B3, B4, B5 and B6 respectively<br>
Colonies with R0011 was denotes B1, B2, B3, B4, B5 and B6 respectively<br>

Revision as of 20:17, 21 October 2010