Team:SDU-Denmark/labnotes2

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(Transformation of cells with gene in plasmid)
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The gene is inserted into a pOT2 plasmid. A plasmid map can be found [http://www.fruitfly.org/about/methods/pOT2vector.html here]:<br><br>
The gene is inserted into a pOT2 plasmid. A plasmid map can be found [http://www.fruitfly.org/about/methods/pOT2vector.html here]:<br><br>
Two of the chlor plates were damaged (one very slightly) and one ampicilin plate was torn up, again. (good thing two were dried). Plates were incubated ON at 37°.<BR><BR>
Two of the chlor plates were damaged (one very slightly) and one ampicilin plate was torn up, again. (good thing two were dried). Plates were incubated ON at 37°.<BR><BR>
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Results: Colonies showed on all plates.<br><br>
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Transformations have been succesfull. If our gene is in there will be tested next week.
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=== Coloni PCR of flhD/C in pSB1C3 ===
=== Coloni PCR of flhD/C in pSB1C3 ===
Start date: 21/07    End date: 21/07<br>
Start date: 21/07    End date: 21/07<br>

Revision as of 15:16, 28 July 2010