Team:SDU-Denmark/labnotes2

From 2010.igem.org

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Gel electrophoresis:<br>
Gel electrophoresis:<br>
[[Image:Team-SDU-Denmark-Miniprep ligering 4 og 9.jpg|600px]]<br><br>
[[Image:Team-SDU-Denmark-Miniprep ligering 4 og 9.jpg|600px]]<br><br>
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Nanodrop:<br>
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<table style="text-align: left; width: 100px;" border="1"
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cellpadding="2" cellspacing="2">
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<tr>
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<td style="vertical-align: top;">Sample ID<br>
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</td>
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<td style="vertical-align: top;">ng/uL<br>
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</td>
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<td style="vertical-align: top;">260/280<br>
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</td>
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<td style="vertical-align: top;">260/230<br>
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</td>
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</tr>
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<tr>
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<td style="vertical-align: top;">miniprep coloni #4.1<br>
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</td>
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<td style="vertical-align: top;">115.68<br>
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</td>
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<td style="vertical-align: top;">1.91<br>
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</td>
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<td style="vertical-align: top;">2.17<br>
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</td>
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</tr>
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<tr>
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<td style="vertical-align: top;">miniprep coloni #4.2<br>
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</td>
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<td style="vertical-align: top;">115.74<br>
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</td>
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<td style="vertical-align: top;">1.91<br>
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</td>
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<td style="vertical-align: top;">2.13<br>
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</td>
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</tr>
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<tr>
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<td style="vertical-align: top;">miniprep coloni #9.1<br>
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</td>
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<td style="vertical-align: top;">131.32<br>
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</td>
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<td style="vertical-align: top;">1.92<br>
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</td>
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<td style="vertical-align: top;">2.23<br>
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</td>
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</tr>
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<tr>
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<td style="vertical-align: top;">miniprep coloni #9.2<br>
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</td>
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<td style="vertical-align: top;">119<br>
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</td>
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<td style="vertical-align: top;">1.93<br>
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</td>
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<td style="vertical-align: top;">2.16<br>
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</td>
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</tr>
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<tr>
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<td style="vertical-align: top;">miniprep coloni #4 pooled<br>
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</td>
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<td style="vertical-align: top;">116.51<br>
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</td>
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<td style="vertical-align: top;">1.92<br>
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</td>
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<td style="vertical-align: top;">2.15<br>
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</td>
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</tr>
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<tr>
 +
<td style="vertical-align: top;">miniprep coloni #9 pooled<br>
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</td>
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<td style="vertical-align: top;">123.94<br>
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</td>
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<td style="vertical-align: top;">1.93<br>
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</td>
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<td style="vertical-align: top;">2.17<br>
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</td>
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</tr>
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</table>
''Analysis:''
''Analysis:''
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We have purified the plasmids, and they are ready for sequentation.<br>
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We have purified the plasmids. The concentrations however are too low for sequentation, so the samples are dried down to reach a conc. of 200ng/uL <br>
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--[[User:Tipi|Tipi]] 08:44, 28 July 2010 (UTC)<br><br>
=== Digestion of flhD/C mut with PstI ===
=== Digestion of flhD/C mut with PstI ===

Revision as of 08:44, 28 July 2010