Team:SDU-Denmark/labnotes2

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(Difference between revisions)
(Amplification of pSB1A2 w. J13002, pSB1A2 w. B0015 and pSB1A2 w. B0034)
(Amplification of pSB1A2 w. J13002)
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--[[User:Pernm07|Pernm07]] 10:06, 22 July 2010 (UTC)
--[[User:Pernm07|Pernm07]] 10:06, 22 July 2010 (UTC)
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===Amplification of pSB1A2 w. J13002===
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===Amplification and purification of pSB1A2 w. J13002===
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<br>
''Experiment done by:'' Pernille <br><br>
''Experiment done by:'' Pernille <br><br>
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''Protocol:'' [https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP1.1]and [https://2010.igem.org/Team:SDU-Denmark/protocols#DNA_extraction_from_gel_.28fermentas.29 DNA extraction] <br><br>
''Protocol:'' [https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP1.1]and [https://2010.igem.org/Team:SDU-Denmark/protocols#DNA_extraction_from_gel_.28fermentas.29 DNA extraction] <br><br>
''Method:'' PCR and DNA extraction <br><br>
''Method:'' PCR and DNA extraction <br><br>
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''Notes:'' The DNA purified in the last experiment was amplified with PRC using VR og VF2 primers and pfu. the normal pfu program was used for running PCR. The PRC product was loaded on a 2% agarose gel. the brick J13002 (promotor+RBS) are 312bp long wich correspond to the visible band on the gel.
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''Notes:'' The DNA purified in the last experiment was amplified with PRC using VR og VF2 primers and pfu. the normal pfu program was used for running PCR. The PRC product was loaded on a 2% agarose gel. the brick J13002 (promotor+RBS) are 312bp long wich correspond to the visible band on the gel.<br><br>
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[[Image:Team-sdu-denmark-Gelextraktionpromotor+rbs.jpg|400px]]
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[[Image:Team-sdu-denmark-Gelextraktionpromotor+rbs.jpg|400px]]<br><br>
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The DNA was extracted and afterwards the DNA concentration was measured on Nanodrop. the concentration was found to 13,02bg/uL. The DNA is saved and afterward it needs to be cut with restriction enzymes.
== Group: Photosensor ==
== Group: Photosensor ==

Revision as of 08:00, 27 July 2010